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相关概念视频

Lysosomal Hydrolases01:22

Lysosomal Hydrolases

3.9K
Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...
3.9K
Lysosomes01:31

Lysosomes

19.3K
Lysosomes are membrane-enclosed spherical sacs derived from the Golgi apparatus. The most important function of the lysosome is degrading macromolecules and biological polymers that are released during membrane trafficking events such as the secretory, endocytic, autophagic, and phagocytic pathways. The degradation is carried out by several hydrolytic enzymes active in an acidic environment of the lysosomal lumen. These acid hydrolases are involved in cellular processes such as cell signaling,...
19.3K

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相关实验视频

Updated: Jul 27, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

2.6K

检测 lysosomal 的活动.

Xin Li1,2, Yanan Sun1, Xiaochen Wang1,2

  • 1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Science, Beijing 100101, China.

Biophysics reports
|June 8, 2023
PubMed
概括
此摘要是机器生成的。

研究人员开发了新的方法来追踪活体中的 lysosome 功能. 这些分析量化了C. elegans中的溶酶成熟,酸度和酶活性,有助于研究细胞平衡.

关键词:
酸性化 酸性化是指酸性化.这里是C. elegans.裂活动的裂活动.Lysosomes 是一个溶解体.成熟 成熟 成熟 成熟 成熟

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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples
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Method for Measuring the Activity of Deubiquitinating Enzymes in Cell Lines and Tissue Samples

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Last Updated: Jul 27, 2025

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
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Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
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The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

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科学领域:

  • 细胞生物学 细胞生物学
  • 器官的功能 器官的功能
  • 恒常状态 (Homeostasis) 是一种恒常状态.

背景情况:

  • 溶解体是重要的器官,负责分解和回收细胞组件.
  • 它们在营养感知和信号传递中起着至关重要的作用,维持细胞和组织平衡 (平衡).
  • 在发育或压力过程中,在体内追踪 lysosomal 属性的变化是具有挑战性的.

研究的目的:

  • 开发和验证用于量化溶酶体性质的新型体内试验.
  • 为了能够研究 lysosomal 功能的变化,以应对生理变化.

主要方法:

  • 使用模型生物C. elegans进行体内研究.
  • 开发了特定的测定方法来测量溶酶体成熟,内部pH值 (酸化) 和酶 (分裂) 活性.
  • 在活体中量化了这些溶酶体参数.

主要成果:

  • 在C. elegans.中成功建立和实施了体内溶解体分析的试验.
  • 证明了这些测试能够检测 lysosomal 活动的变化.
  • 展示了测试在发育期间和应激状态下研究 lysosome 功能的实用性.

结论:

  • 开发的试验为酶体动态的体内调查提供了一个强大的工具.
  • 这些方法有助于人们了解 lysosomal 功能如何对生物体的恒常性有所贡献.
  • 这些测试适用于研究各种生物环境中的溶酶体变化,包括发育和应激反应.