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相关概念视频

Preparation of Carboxylic Acids: Overview01:31

Preparation of Carboxylic Acids: Overview

2.7K
There are various methods for the preparation of carboxylic acids. For example, oxidation of primary alcohols or aldehydes using strong oxidizing agents results in a carboxylic acid.  Aldehydes can also be oxidized in the presence of mild oxidizing agents.
2.7K
Preparation of Aldehydes and Ketones from Nitriles and Carboxylic Acids01:24

Preparation of Aldehydes and Ketones from Nitriles and Carboxylic Acids

3.6K
Although it is possible to reduce a carboxylic acid to an aldehyde, strong reducing agents, like lithium aluminum hydride (LAH), prohibit a controlled reduction, instead causing the generated aldehyde to instantly over-reduce to a primary alcohol.
Reducing carboxylic acid derivatives like acyl chlorides (RCOCl), esters (RCO2R′), and nitriles (RCN) using milder aluminum hydride agents like lithium tri-tert-butoxyaluminum hydride [LiAlH(O-t-Bu)3] and diisobutylaluminum hydride [DIBAL-H]...
3.6K
Preparation of Epoxides03:00

Preparation of Epoxides

7.9K
Overview
Epoxides result from alkene oxidation, which can be achieved by a) air, b) peroxy acids, c) hypochlorous acids, and d) halohydrin cyclization.
Epoxidation with Peroxy Acids
Epoxidation of alkenes via oxidation with peroxy acids involves the conversion of a carbon–carbon double bond to an epoxide using the oxidizing agent meta-chloroperoxybenzoic acid, commonly known as MCPBA. Since the O–O bond of peroxy acids is very weak, the addition of electrophilic oxygen of...
7.9K
Preparation of Nitriles01:12

Preparation of Nitriles

2.1K
One of the common methods to prepare nitriles is the dehydration of amides. This method requires strong dehydrating agents like phosphorous pentoxide or boiling acetic anhydride for converting amides to nitriles. Another reagent namely, thionyl chloride also accomplishes the dehydration of amides, where amide acts as a nucleophile. The first step of the mechanism involves the nucleophilic attack by the amide on the thionyl chloride to form an intermediate. In the next step, the electron pairs...
2.1K
Preparation of Acid Anhydrides01:07

Preparation of Acid Anhydrides

3.3K
One of the methods for preparing symmetrical or unsymmetrical acid anhydrides involves the treatment of acid chlorides with the sodium salt of carboxylic acids. The reaction proceeds via a nucleophilic acyl substitution.
The carboxylate ion acts as a nucleophile that attacks the carbonyl carbon of the acid chloride to form a tetrahedral intermediate. Subsequently, the re-formation of the carbonyl group with the loss of the chloride ion as a leaving group leads to the formation of an acid...
3.3K
Preparation of Amides01:29

Preparation of Amides

3.2K
Amides are synthesized by treating carboxylic acids with amines in the presence of dehydrating agents like dicyclohexylcarbodiimide (DCC).
The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
Subsequently, the amine acts as a nucleophile that attacks the acylating agent to form a tetrahedral intermediate. In the...
3.2K

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相关实验视频

Updated: Jul 27, 2025

Preparation of High-Quality Fermented Fish Product
00:05

Preparation of High-Quality Fermented Fish Product

Published on: August 23, 2019

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准备的准备的准备.

Yuqin Wang1,2, Pingping Fan1,2, Shanyu Zhang1,2

  • 1State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

Biophysics reports
|June 8, 2023
PubMed
概括
此摘要是机器生成的。

我们提出了一个简化的协议,用于准备Mycobacterium smegmatis porin A (MspA) 纳米孔,这对于纳米孔测序和纳米反应感应至关重要. 这种方法可以为单分子传感应用产生高质量的MspA蛋白.

关键词:
关联性净化净化方法在MspA纳米孔中.Prokaryotic 的表达方式单个分子感应 单个分子感应

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Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM
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Preparation of Naringenin Solution for In Vivo Application
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Preparation of High-Quality Fermented Fish Product
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Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM
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Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy SEM

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Preparation of Naringenin Solution for In Vivo Application
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Preparation of Naringenin Solution for In Vivo Application

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科学领域:

  • 生物物理学的生物物理.
  • 纳米技术 纳米技术
  • 分子生物学分子生物学

背景情况:

  • 由于其狭窄的狭窄,Mycobacterium smegmatis porin A (MspA) 提供了高空间分辨率的传感.
  • MspA非常适合纳米孔测序和纳米反应感应,但尚未商业化.
  • 现有的MSPA制备方法复杂且难以获得.

研究的目的:

  • 开发一个简化和可访问的MspA准备协议.
  • 为了证明准备好的MspA纳米孔对于单分子传感的实用性.
  • 为了使基于MspA的纳米反应式传感器的开发.

主要方法:

  • 为MspA蛋白制剂开发了一个简单的协议.
  • 该协议的重点是实现完全寡合化MspA蛋白的高产量.
  • 使用固定式ssDNA寡核酸歧视来证明MspA纳米孔功能.

主要成果:

  • 该方案在每升培养物中产生大约10毫克的完全寡合化MspA蛋白.
  • 准备好的MspA纳米孔成功地区分了不移动的ssDNA寡核酸.
  • 该协议与MspA突变体兼容,促进了传感器的开发.

结论:

  • 建立了一个简化的MspA制备协议,克服了商业不可用性.
  • 该协议为纳米孔测序和纳米反应感应提供了可靠的MSPA来源.
  • 这项工作有助于创建基于MspA的多种纳米传感器,用于各种应用.