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Updated: Jul 27, 2025

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Preparation of High-Quality Fermented Fish Product
Published on: August 23, 2019
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准备的准备的准备
Yuqin Wang1,2, Pingping Fan1,2, Shanyu Zhang1,2
1State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Biophysics reports
|June 8, 2023
概括
我们提出了一个简化的协议,用于准备Mycobacterium smegmatis porin A (MspA) 纳米孔,这对于纳米孔测序和纳米反应感应至关重要. 这种方法可以为单分子传感应用产生高质量的MspA蛋白.
科学领域:
- 生物物理学的生物物理.
- 纳米技术 纳米技术
- 分子生物学分子生物学
背景情况:
- 由于其狭窄的狭窄,Mycobacterium smegmatis porin A (MspA) 提供了高空间分辨率的传感.
- MspA非常适合纳米孔测序和纳米反应感应,但尚未商业化.
- 现有的MSPA制备方法复杂且难以获得.
研究的目的:
- 开发一个简化和可访问的MspA准备协议.
- 为了证明准备好的MspA纳米孔对于单分子传感的实用性.
- 为了使基于MspA的纳米反应式传感器的开发.
主要方法:
- 为MspA蛋白制剂开发了一个简单的协议.
- 该协议的重点是实现完全寡合化MspA蛋白的高产量.
- 使用固定式ssDNA寡核酸歧视来证明MspA纳米孔功能.
主要成果:
- 该方案在每升培养物中产生大约10毫克的完全寡合化MspA蛋白.
- 准备好的MspA纳米孔成功地区分了不移动的ssDNA寡核酸.
- 该协议与MspA突变体兼容,促进了传感器的开发.
结论:
- 建立了一个简化的MspA制备协议,克服了商业不可用性.
- 该协议为纳米孔测序和纳米反应感应提供了可靠的MSPA来源.
- 这项工作有助于创建基于MspA的多种纳米传感器,用于各种应用.
相关概念视频
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Reducing carboxylic acid derivatives like acyl chlorides (RCOCl), esters (RCO2R′), and nitriles (RCN) using milder aluminum hydride agents like lithium tri-tert-butoxyaluminum hydride [LiAlH(O-t-Bu)3] and diisobutylaluminum hydride [DIBAL-H]...
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Epoxides result from alkene oxidation, which can be achieved by a) air, b) peroxy acids, c) hypochlorous acids, and d) halohydrin cyclization.
Epoxidation with Peroxy Acids
Epoxidation of alkenes via oxidation with peroxy acids involves the conversion of a carbon–carbon double bond to an epoxide using the oxidizing agent meta-chloroperoxybenzoic acid, commonly known as MCPBA. Since the O–O bond of peroxy acids is very weak, the addition of electrophilic oxygen of...
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The carboxylate ion acts as a nucleophile that attacks the carbonyl carbon of the acid chloride to form a tetrahedral intermediate. Subsequently, the re-formation of the carbonyl group with the loss of the chloride ion as a leaving group leads to the formation of an acid...
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The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
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