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Updated: Jul 27, 2025

Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone
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Author Spotlight: An Alternative Approach to Protein Quantification by Bradford Assay Using a Smartphone

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通过紫外线吸收和布拉德福德试验量化核蛋白复合体

Jiang Chen1,2,3, Hao Luo1,3, Mei Tao1

  • 1Key Laboratory of Environmental and Applied Microbiology, Chengdu Institute of Biology, Chinese Academy of Sciences; Key Laboratory of Environmental Microbiology of Sichuan Province, Chengdu 610041, China.

Biophysics reports
|June 8, 2023
PubMed
概括

核蛋白复合体的量化是至关重要的. 这项研究提出了两种准确的方法,即修改的紫外线吸收测试和布拉德福德测试,用于测量这些复合体内的蛋白质度.

关键词:
布拉德福德测定方法核蛋白质复合体是一个核蛋白质复合体.量化 量化 量化 量化基于紫外线衍生配方的方法.

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Real Time RT-PCR02:57

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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