微生物电合成与微生物电合成
Santiago T Boto1,2, Bettina Bardl1, Falk Harnisch3
1Leibniz Institute for Natural Product Research and Infection Biology - Hans Knöll Institute (Leibniz-HKI) Jena Germany miriam.rosenbaum@leibniz-hki.de.
概括
微生物电合成 (MES) 使用作为Clostridium ljungdahlii的主要电子来源,增强生长和生物合成. 这项研究阐明了电子转移机制,改善了MES工艺工程和产品产量.
科学领域:
- 微生物的电合成.
- 电子生理学 电子生理学
- 生物化学工程 生物化学工程
背景情况:
- 微生物电合成 (MES) 为二氧化碳回收成有价值的有机化合物提供了一个有前途的途径.
- 对微生物细胞外电子转移 (EET) 的有限理解阻碍了MES的发展.
- 通过直接或间接的路径消耗电子的Clostridium ljungdahlii仍然不清楚.
研究的目的:
- 为了阐明Clostridium ljungdahlii在电自otrophic MES中的主导电子源.
- 调查的可用性对C. ljungdahlii生活方式和代谢活动的影响.
- 优化MES流程,以增强生长,生物合成和产品形成.
主要方法:
- 使用了与Clostridium ljungdahlii一起使用的电自otrophic MES.
- 控制的可用性作为电子源.
- 监测浮游生物和生物膜的形成,细胞密度,代谢活性和产品标位.
主要成果:
- 阴极被证实是MES中C. ljungdahlii的主导电子来源.
- 的可用性决定了浮游生物与生物膜的生活方式,有利于浮游生物的生长.
- 优化的条件产生了高的乙酸标位 (6.06 g L-1) 和生产率 (0.11 g L-1 d-1).
- 首次观察到显著的甘氨酸 (0.39克L-1) 和乙醇胺 (0.14克L-1) 的产生.
结论:
- 了解C. ljungdahlii的电生理学对于推动MES的发展至关重要.
- 与以前的方法相比,通过介导的MES可以实现更好的生长和生物合成.
- 这项研究为改善MES研究中的生物工艺设计和工程提供了基础.
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