对抗微生物对抗菌的评估.
Gamze Göger1, Deniz Yüksel2, Fatih Göger3
1Faculty of Pharmacy, Department of Pharmacognosy, Afyonkarahisar Health Sciences University, Afyonkarahisar, Türkiye.
概括
三重精精提取物显示出对尿路病原体的潜在抗菌作用. 水提取物在高度下对C. elegans无毒,输液提取物在体内表现出抗感染性质.
科学领域:
- 民族植物学 民族植物学
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
背景情况:
- 在土耳其,Tripleurospermum callosum传统上用于尿道和呼吸道疾病.
- 尿路感染 (UTI) 是一个重大的公共卫生问题,通常是由耐药性病原体引起的.
研究的目的:
- 研究Tripleurospermum callosum提取物对常见的尿路病原体的体外和体内抗菌活性.
- 用C. elegans作为模型生物来确定这些提取物的无毒度.
- 分析提取物的植物化学成分.
主要方法:
- 从T. callosum的上空部分中制备的输液,蒸和96%的乙醇提取物.
- 在体外针对大肠杆菌,金黄色葡萄球菌,Pseudomonas aeruginosa,Klebsiella aerogenes和Candida albicans的抗菌分析.
- 在体内进行抗微生物测定和使用Caenorhabditis elegans的毒性评估.
- 使用液体染色学-质谱学 (LC-MS/MS) 的植物化学分析.
主要成果:
- 水提取物在C. elegans中表现出非毒性,其度在312至5000微克/毫升之间.
- 在C. elegans. 中,96%的乙醇提取物在312μg/mL时无毒.
- 输液提取物在体内表现出在312-5000μg/ml的度下对格拉姆阴性细菌具有抗感染作用.
结论:
- 三重精结骨提取物具有对尿路系统病原体有前途的非毒性和抗感染性.
- 对T. callosum的进一步研究可能会导致针对尿路感染的新型治疗剂.
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