在HTB-4细胞中,细胞因子的概况与HTB-4细胞的细胞因子概况有关
Snehashis Koley1, Mandira Mukherjee1
1Department of Biochemistry & Medical Biotechnology, School of Tropical Medicine, Kolkata, 700073, India.
Future microbiology
|June 8, 2023
概括
孕妇和非孕妇患者的泌尿病原性大肠杆菌 (UPEC) 呈现出明显的抗微生物耐药性和毒性因子表达. 细胞因子概况与毒性基因相关,为尿路感染的治疗策略提供信息.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 泌尿病原性大肠杆菌 (UPEC) 是尿路感染 (UTI) 的常见原因之一.
- 了解孕妇和非孕妇的UPEC特征对于有效治疗至关重要.
- 抗微生物耐药性 (AMR) 和毒性因子表达影响疾病严重程度和宿主反应.
研究的目的:
- 从怀孕和非怀孕的患者中描述UPEC.
- 研究AMR,毒性因子和宿主细胞因子反应之间的关系.
- 为开发针对UPEC感染的向疗法提供信息.
主要方法:
- 抗生素敏感性测试和尿细胞粘附性测试.
- 聚合酶链反应 (PCR) 和实时PCR用于基因表达分析.
- 在实验室感染模型使用HTB-4尿细胞.
主要成果:
- 来自非怀孕患者的UPEC表现出更高的抗菌素耐药性.
- 在非怀孕的UPEC中,观察到特定的毒性基因 (hlyA,papC) 和细胞因子 (TGF-β,GCSF) 之间存在显著的相关性.
- 在孕妇患者的UPEC中发现了fimH和细胞因子 (IFN-γ,IL-1β,IL-17A) 之间的明显相关性.
结论:
- 细胞因子表达特征与UPEC中的毒性基因表达有关.
- 这些相关性在从孕妇和非孕妇中分离的UPEC之间有所不同.
- 在UPEC感染的治疗策略中,应考虑AMR和细胞因子概况.
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