开发一个的发展
Marco Calvigioni1, Adelaide Panattoni1, Francesco Biagini2,3
1Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.
Microbiology spectrum
|June 8, 2023
概括
涂有真的人工肠道模型成功地模仿了与粘液相关的肠道微生物. 这一进步使我们能够更好地研究益生菌和药物等因素如何影响这些关键的,专门的肠道细菌.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 生物材料科学 生物材料科学
背景情况:
- 肠道微生物群的粘液相关层在组成和功能上与光线社区有显著的不同.
- 在体外研究这些特定的微生物种群是具有挑战性的,但对于理解肠道健康至关重要.
- 现有的体外模型往往无法充分代表粘液粘附的微生物.
研究的目的:
- 开发和验证一个体外模型,专门支持和选择粘液相关的肠道微生物群落.
- 在三维模型中研究粘膜在塑造殖民肠道微生物群组成中的作用.
- 建立一个可靠的平台来研究外部因素对粘液粘附微生物的影响.
主要方法:
- 使用电凝支架,有或没有粘素补充,作为一个三维体外肠道模型.
- 用人类便样本进行注射的支架,以建立肠道微生物生物膜.
- 微生物粘附,生长,社区组成和生物多样性的比较,在粘素涂层和非涂层的支架之间随时间推移.
主要成果:
- 既有粘素涂层和无涂层的支架都支持稳定,长期的肠道微生物生物膜,具有可比的细菌负载和生物多样性.
- 涂有菌素的支架显示了关键的粘膜相关细菌的显著丰富,包括Akkermansia,Lactobacillus和Faecalibacterium.
- 该模型成功地选择了已知在体内粘附于肠粘液层的微生物.
结论:
- 菌素在塑造肠道微生物群落中起着至关重要的作用,即使在人造体外系统中也是如此.
- 使用粘液涂层的电凝结构开发的体外模型是研究粘液粘附微生物群落的有效工具.
- 该模型为评估营养素,益生菌,病原体和药物对特定肠道微生物群的影响提供了一个有希望的平台.
相关概念视频
01:27Development of the Heart
1.1K
The development of the human heart, a crucial organ, commences from the mesoderm on the 18th or 19th day after fertilization. This process initiates in the cardiogenic area, a group of mesodermal cells at the embryo's head end, which evolves into elongated strands known as cardiogenic cords. These cords undergo a transformation to form hollow-centered endocardial tubes.
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart...
As the embryo undergoes lateral folding, these paired tubes approach each other, merging into a single primitive heart...
1.1K
01:22Development of Immunocompetence
366
The initiation of cell-mediated immunity can be observed as early as the third month of fetal growth, with active antibody-mediated immunity following approximately one month later.
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
The initial cells that migrate from the fetal thymus settle within the skin and epithelial tissues lining the mouth, digestive tract, and in females, the uterus and vagina. These cells, including skin-based dendritic cells, serve as antigen-presenting cells, playing a key role in T cell activation.
Subsequent T...
366
01:15Development of the Lymphatic System
834
The development of lymphatic tissues and vessels in embryonic life begins around the fifth week. These structures originate from the mesoderm layer, with lymph sacs emerging from developing veins.
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
The first lymph sacs to form are the paired jugular lymph sacs located at the junction of the internal jugular and subclavian veins. From these sacs, lymphatic capillary plexuses extend to the thorax, upper limbs, neck, and head, eventually forming lymphatic vessels. Each jugular lymph sac maintains a...
834
01:28Preclinical Development: Overview
4.5K
Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...
4.5K
01:43Sustainable Development
13.4K
As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
13.4K
01:27Introduction to Developmental Psychology
311
Developmental psychology explores the changes and continuities in human abilities throughout life, encompassing physical, cognitive, linguistic, and social dimensions. Human development is not restricted to growth, but includes aspects of decline, particularly in physical abilities as individuals age. Developmental psychologists seek to understand how people change as they age and how their mental and social skills evolve.
Developmental Milestones
A key concept in developmental psychology is...
Developmental Milestones
A key concept in developmental psychology is...
311