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相关概念视频

Genomics02:02

Genomics

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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Genome Annotation and Assembly03:36

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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Next-generation Sequencing03:00

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Maxam-Gilbert Sequencing01:05

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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相关实验视频

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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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完整的基因组序列的完整基因组序列

Gyungcheon Kim1, Jin Young Park2, Jin Nam Kim3

  • 1Department of Food Science and Biotechnology, College of Life Science, Sejong University, Seoul, South Korea.

Microbiology resource announcements
|June 8, 2023
PubMed
概括

我们测序了Phenylobacterium sp.的完整基因组. 菌株NIBR 498073,从韩国潮平面沉积物中分离出来. 这项分析为这种细菌物种提供了基础的基因组数据.

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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
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Novel Sequence Discovery by Subtractive Genomics
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科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.

背景情况:

  • 类细菌 (Phenylobacterium) 是一种在各种环境中发现的细菌属.
  • 基因组数据对于理解细菌多样性和功能至关重要.

研究的目的:

  • 报告Phenylobacterium sp.的完整基因组序列. 这种菌株是NIBR 498073.
  • 为这个菌株提供一个基础的基因组资源.

主要方法:

  • 在Phenylobacterium sp.的全基因组测序. 这种菌株是NIBR 498073.
  • 使用 Prokaryotic Genome Annotation Pipeline (PGAP) 进行基因组注释.

主要成果:

  • 这是Phenylobacterium sp.的完整基因组序列. 已经确定了NIBR 498073菌株.
  • 基因组包括一个由4,289,989个基对组成的单个圆形染色体.
  • 标注预测了4,160个编码蛋白质的基因,47个tRNA,6个rRNA和3个非编码RNA.

结论:

  • 该基因组序列为Phenylobacterium sp.提供了一个全面的遗传蓝图. 这种菌株是NIBR 498073.
  • 这些数据将有助于未来研究这种细菌的生物学和生态作用.