用微生物生物质,分子和酶生产的乳清基侧流的价值化
Anna Poladyan1,2, Karen Trchounian3,4, Ani Paloyan5
1Department of Biochemistry, Microbiology, and Biotechnology, Yerevan State University, Yerevan, Armenia, 1 A. Manoukian Str, 0025, Yerevan, Armenia. apoladyan@ysu.am.
Applied microbiology and biotechnology
|June 8, 2023
概括
乳制品行业的侧流,如乳清,可以有效地用于微生物培养和有价值的化合物生产. 这项研究表明,在经过处理的乳清上,成功地使用工程Escherichia coli和Ralstonia eutropha产生生物质和气.
科学领域:
- 生物技术和工业微生物学
- 生物反应器工程和过程优化
- 可持续化学和生物基产品
背景情况:
- 乳制品行业的副流,如甜乳糖 (SW) 和酸乳糖 (AW),是富含营养来源,具有培养微生物的潜力.
- 大肠杆菌和Ralstonia eutropha是产生酶,重组蛋白和分子 (H2) 的重要生物技术兴趣的微生物.
- 从工业副产品中开发具有成本效益和可持续的培养基对于推动生物生产至关重要.
研究的目的:
- 调查使用预处理乳清作为大肠杆菌和Ralstonia eutropha的培养基的可行性.
- 评估β-葡萄糖酶处理对乳清成分的影响及其对微生物生长和产品形成的影响.
- 评估使用乳清的选定微生物菌株的生物质,酶 (Hyd) 活性和分子 (H2) 的产生.
主要方法:
- 甜乳糖 (SW) 和酸乳糖 (AW) 的预处理,包括过,稀释和pH调整,有或没有古热稳定β-葡萄糖酶.
- 在受控条件下培养大肠杆菌BW25113和Ralstonia eutropha H16 (大肠杆菌的温度为37°C,pH值为7.5;大肠杆菌的温度为30°C,pH值为7.0).
- 监测生长参数,包括氧化降低潜力 (ORP),pH,特异生长率和生物质形成 (OD600);测量酶活性和H2生产.
主要成果:
- 大肠杆菌和R. eutropha在过和未过的乳清上都表现出良好的发酵生长,pH和ORP测量表明栽培成功.
- 在乳清上,R. eutropha获得了最大的细胞产量 (OD600 4.0) 和H2-氧化Hyd活性,与对照果糖-最小盐介质相当.
- 工程E. coli表现出显著的生物质生产和持续的H2产量 (约. 5 mmol/L,累计94 mL/g干乳清) 在β-葡萄糖酶处理后,证明了依赖于H3的H2生产.
结论:
- 乳清,特别是当用热稳β-葡萄糖酶处理乳糖水解时,是生产微生物生物质和生物催化剂的经济可行的基质.
- 该研究证实了使用乳制品侧流作为生物技术应用 (包括H2生产) 的可持续原料的潜力.
- 这项研究为有效的乳清价值化和利用大肠杆菌和R. eutropha等微生物平台开发高效的生物过程开辟了新的途径.
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