富含三糖脂蛋白介导的聚合物点用于多式成像 护骨间 棕色脂肪组织毛细血管
Jingru Li1, Yixiao Guo1, Panting Ren1
1Shanghai Med-X Engineering Center for Medical Equipment and Technology, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, P. R. China.
ACS applied materials & interfaces
|June 8, 2023
概括
聚合物点 (Pdots) 可以通过与富含甘油三蛋白的脂蛋白 (TRL) 结合来描绘棕色脂肪组织 (BAT) 的活性. 裸体Pdots在毛细血管内皮细胞中显示出高吸收率,反映了用于代谢障碍研究的BAT活性.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 代谢障碍 代谢障碍 代谢障碍
- 纳米技术纳米技术
背景情况:
- 棕色脂肪组织 (BAT) 是代谢障碍的关键目标.
- [18F]FDG-PET通常用于BAT成像,但具有局限性.
- 对于先进的BAT成像,需要新的功能探针.
研究的目的:
- 在BAT中阐明聚合物点 (Pdot) 成像的机制.
- 开发Pdots用于敏感检测BAT活动.
- 在体内量化富含甘油三脂脂蛋白 (TRL) 的摄取量.
主要方法:
- 研究了Pdot与TRL的结合亲和力.
- 评估Pdot积累在骨间棕色脂肪组织 (iBAT) 毛细体内皮细胞 (ECs).
- 将裸体Pdots与PSMAC-Pdots和PEG-Pdots进行比较.
- 使用多式联络Pdots进行体内成像.
主要成果:
- Pdots与TRL结合,并选择性地积聚在IBAT毛细血管EC中.
- 裸体Pdots表现出良好的脂性和快速吸收 (94%在5分钟内).
- 在iBAT中积累的Pdot与BAT活动相关,特别是在冷刺激后.
结论:
- 在iBAT中积累的Pdot反映了BAT活动.
- Pdots提供了一种敏感的方法来检测和量化iBAT中的TRL吸收.
- 这种方法推进了用于代谢障碍研究的多式成像.
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