纯粹的,Ni和Zn注的CuSe纳米颗粒:一种抗菌,抗氧化和细胞毒性研究
Sefali R Patel1, Sunil H Chaki1,2, Ranjan Kr Giri1
1P. G. Department of Physics, Sardar Patel University, Vallabh Vidyanagar 388120, Gujarat, India.
ACS applied bio materials
|June 8, 2023
概括
这项研究使用化学共降合成了原始和杂的化铜 (CuSe) 纳米粒子. 化纳米颗粒表现出不同的抗微生物,抗氧化剂和细胞毒性作用,原始CuSe显示对肺癌细胞的有效性.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 化学 化学 化学
背景情况:
- 化铜 (CuSe) 纳米颗粒对各种应用有兴趣.
- (Ni) 和 (Zn) 等过渡金属的注可以改变纳米粒子的特性.
- 了解合CuSe的合成和表征对于进一步开发至关重要.
研究的目的:
- 合成原始和Ni-或Zn-合的CuSe纳米粒子.
- 描述合成纳米颗粒的结构,形态和稳定性质.
- 评估合成纳米颗粒的抗菌,抗氧化和细胞毒性活动.
主要方法:
- 纳米粒子合成的化学共降.
- 射线能量分散谱 (XEDS) 用于静电测量和元素映射.
- 用X射线衍射 (XRD) 来识别相位和晶体结构.
- 场辐射显微镜 (FEM) 用于形态学和选区电子衍射 (SAED) 用于结晶性.
- 动态光散射 (DLS) 用于尺寸分布和泽塔电位测量以获得稳定性.
- 对五种细菌菌株进行抗菌检测.
- 2,2-二-1-皮克利水 (DPPH) 扫除对抗氧化活性的测试.
- 盐水杀伤性测定体内细胞毒性.
- 在体外细胞毒性测定使用人类肺癌细胞系 (A549).
主要成果:
- 合成的纳米粒子是近立体的,具有六角结构的单相,球形.
- 与Ni和Zn合的CuSe纳米颗粒显示出不同程度的抗菌活性.
- 纯 CuSe 纳米颗粒显示出对 A549 肺癌细胞 (IC50 = 488 μg/mL) 的最有效的细胞毒性.
- 10% Ni-和10% Zn-doped CuSe纳米颗粒在盐水的测试中显示出100%的死亡率.
- 维生素C的抗氧化活性最高,Ni-化CuSe的活性最低.
结论:
- 化学共降是一种可行的合成合CuSe纳米颗粒的方法.
- 兴奋剂会影响CuSe纳米颗粒的稳定性和生物活性.
- 纯 CuSe 纳米颗粒对肺癌治疗有前途,而合版本可能在抗菌或其他细胞毒性疗法中有应用.
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