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基于热稳定的伴侣的多生物合成:恩福维尔提德模型产品质量和与协议相关的杂质

Vladimir Zenin¹, Andrey Tsedilin¹, Maria Yurkova¹

¹Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russian Federation.

|June 8, 2023

概括

大型生物合成为Enfuvirtide提供了一种新的生产方法,Enfuvirtide是艾滋病毒治疗的关键. 虽然符合质量标准,但与化学合成Enfuvirtide相比,观察到治疗活性的微小差异.

科学领域:

生物技术是生物技术.
制药合成制药合成
生物化学生物化学

背景情况:

像Enfuvirtide这样的大型治疗对于艾滋病毒治疗至关重要.
传统的化学合成对大型的生产提出了挑战.
生物技术方法提供了替代的制造途径.

研究的目的:

为了评估生物合成产生的恩福维的质量和杂质概况.
为了比较生物合成Enfuvirtide和化学合成Enfuvirtide的特征.
评估使用基于热稳定的伴侣系统用于大型制造的可行性.

主要方法:

基于热稳定的伴侣生物合成系统.
液体染色学-质谱学 (LC-MS) 用于杂质分析.
循环二重化 (CD) 光谱用于结构比较.
使用MT-4细胞进行HIV感染抑制测定.

主要成果:

生物合成Enfuvirtide符合质量要求,具有较低的内毒素 (1.06 EU/mg) 和宿主细胞蛋白 (HCP) 水平 (5.58 ppm).
通过LC-MS分析,确定和量化了与工艺相关的杂质.
治疗活性 (IC50) 显示出差异 (0.0453μM与0.0180μM),归因于非化C端.

结论:

类生物合成是一种可行的替代方法,可以生产像Enfuvirtide这样的治疗性.
微小的结构差异可能会影响生物活动,需要进一步调查.
开发的系统显示了大规模治疗性制造的潜力.

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