相关实验视频
Updated: Jul 27, 2025
22:27
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
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通过同时单细胞Hi-C和RNA-seq将基因组结构与功能联系起来
Zhiyuan Liu1,2, Yujie Chen1,2, Qimin Xia1,2
1Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, Peking University, Beijing, China.
Science (New York, N.Y.)
|June 8, 2023
概括
一种新的方法,即同时使用Hi-C和RNA-seq (HiRES),将3D基因组结构与单细胞中的基因表达联系起来. 这揭示了细胞发育过程中的染色体重新连接是如何先于基因激活的.
科学领域:
- 基因组学和分子生物学
- 发育生物学
- 表观遗传学
背景情况:
- 单细胞染色体构成捕获技术已经显著进步.
- 缺乏同时分析染色体结构和基因表达的方法.
研究的目的:
- 开发和应用一种用于同时单细胞染色体结构和基因表达特征的新型试验.
- 在小鼠胚胎发育过程中研究3D基因组结构的动态.
主要方法:
- 开发同时使用的Hi-C和RNA-seq测定方法 (HiRES).
- 将HiRES应用于成千上万个小鼠胚胎中的单细胞.
主要成果:
- 单细胞3D基因组结构在发育过程中表现出细胞类型特异性,受细胞周期和发育阶段的影响.
- 伪时光分析显示在转录激活之前广泛的染色体重新连接.
- 已确定的染色体相互作用与谱系规范中的转录控制和细胞功能密切相关.
结论:
- 通过HiRES测定,可以同时分析单细胞层面的基因结构和基因表达.
- 染色体重新连接在发育过程中对基因表达和细胞命运起着关键作用.
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