一个对比研究的转录调节机制的细胞染色体P450的研究
Yun Huang1, Junyue Zheng1, Peizhuo Wu1
1Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing 100193, China.
Journal of agricultural and food chemistry
|June 8, 2023
概括
转录因子Ubx和Br调节细胞染色体P450的CYP6B7表达,调解Helicoverpa armigera中的芬瓦莱拉特耐药性. 这项研究确定了CYP6B7促进体中关键的调节部位.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞染色体P450 (CYP6B7) 与Helicoverpa armigera中的芬瓦莱拉特耐药性有关.
- 了解CYP6B7的调节对于管理抗虫剂耐药性至关重要.
研究的目的:
- 为了研究Helicoverpa armigera.中CYP6B7的调节机制.
- 阐明特定促进子区域和转录因子在芬瓦莱拉特耐药性中的作用.
主要方法:
- 对抗性和易受性菌株之间的CYP6B7促进体序列的比较分析.
- 促进体区域的局部定向突变和记者基因测试.
- 转录因子 Ubx 和 Br. 的基因沉默 (敲除)
主要成果:
- 在耐药和敏感菌株之间的CYP6B7促进体中发现了七种基差异 (M1-M7).
- 在M3,M4和M7位点的突变显著降低了芬瓦莱拉特诱导的记者基因活性.
- 分别在M3和M7结合的转录因子Ubx和Br在耐药菌株中过度表达.
- 击败Ubx和Br抑制了CYP6B7的表达,增加了芬瓦莱拉特的敏感性.
结论:
- 转录因子Ubx和Br在调节CYP6B7的表达中起着至关重要的作用.
- 已识别的促进子位点 (M3,M4,M7) 是参与芬瓦莱拉特耐药性的关键调控元素.
- 准Ubx,Br或CYP6B7可能是克服Helicoverpa armigera.fenvalerate耐药性的一个策略.
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