在SM22α表达细胞中的缺氧诱导因子-1α调节了膜化
Elizabeth A Barnes1, Carsten Knutsen1,2, Alida Kindt3
1Division of Pulmonary, Asthma, and Sleep Medicine, Center for Excellence in Pulmonary Biology, and.
概括
在特定的肺细胞中,缺氧诱导因子-1α (HIF-1α) 对于出生后发育健康的气膜和血管至关重要. 它的缺失导致肺部结构和功能受损,类似于支气管肺功能障碍症.
科学领域:
- 肺部医学 肺部医学
- 发展生物学 发展生物学
- 分子遗传学 分子遗传学
背景情况:
- 支气管肺功能障碍症 (BPD) 是早产婴儿常见的慢性肺部疾病,其特征是肺膜发育受损.
- 缺氧诱导因子-1α (HIF-1α) 在肺发育中的作用,特别是在特定的细胞类型中,尚未完全理解.
研究的目的:
- 调查HIF-1α在产后肺膜化和血管生成中的细胞特异性作用.
- 为了确定SM22α表达介质细胞中的HIF-1α是否调解肺部发育.
主要方法:
- 使用Cre-lox技术生成细胞特异的HIF-1α淘汰小鼠 (SM22α-HIF-1α-/-) .
- 利用单细胞RNA测序来识别表达SM22α的细胞亚型.
- 在淘汰赛小鼠和早产婴儿样本中评估肺部结构,血管化和血管蛋白-2表达.
主要成果:
- 在SM22α表达细胞中删除HIF-1α导致气泡较少和较大,持续到成年.
- 在淘汰赛小鼠中观察到微血管密度降低,弹性组织和肺血管分支.
- 缺少HIF-1α的肺血管光滑肌细胞显示血管蛋白-2的表达减少,损害了血管生成.
结论:
- 特定于SM22α的HIF-1α表达对于适当的产后肺膜化和外周血管生成至关重要.
- HIF-1α可能通过调节血管光滑肌细胞中的血管蛋白-2 表达来促进膜化.
- 研究结果表明,通过调节HIF-1α信号传递,BPD是一种潜在的治疗点.
更多相关视频
14:48Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
Published on: March 21, 2021
5.2K
10:49Efficient Transcriptionally Controlled Plasmid Expression System for Investigation of the Stability of mRNA Transcripts in Primary Alveolar Epithelial Cells
Published on: March 6, 2020
6.1K
相关概念视频
Acute Respiratory Failure-II
291
Type I Respiratory Failure, or hypoxemic respiratory failure, occurs when the partial pressure of oxygen (PaO2) in arterial blood falls below 60 mmHg while breathing room air without a corresponding increase in arterial carbon dioxide levels (PaCO2). This condition highlights a significant impairment in the lungs' capacity to oxygenate the blood.
The underlying physiological abnormalities that contribute to hypoxemic respiratory failure include:
The underlying physiological abnormalities that contribute to hypoxemic respiratory failure include:
291
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Adaptive Mechanisms in Cancer Cells
5.8K
Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
5.8K
Hypoxia
1.1K
Hypoxia is a medical condition characterized by an inadequate oxygen supply to body tissues. It typically manifests as a bluish discoloration of the skin and mucosae, especially in fair-skinned individuals, when hemoglobin (Hb) saturation drops below 75%.
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
Types of Hypoxia
There are four primary types of hypoxia, each resulting from a different cause:
1. Anemic hypoxia: This type occurs due to insufficient oxygen delivery caused by a lack of red blood cells (RBCs) or RBCs with abnormal or...
1.1K
Alveoli and Alveolar Ducts
2.9K
The respiratory zone of the human body, which stands in contrast to the conducting zone, comprises the structures that actively participate in the exchange of gases. The initiation of this zone is marked by the terminal bronchioles converging into respiratory bronchioles, the tiniest bronchiole classification. The respiratory bronchioles give way to the alveolar ducts that opens into a congregation of alveoli. Actively involved in gas exchange, alveoli resemble tiny sacs similar to clusters of...
2.9K
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
