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循环螺旋B减轻了前炎性细胞死亡,并改善了创伤性脊髓损伤后的功能恢复

Yu Xu¹, Yibo Geng², Hui Wang²

¹Department of Orthopaedics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, 325027, China; Zhejiang Provincial Key Laboratory of Orthopaedics, Wenzhou, 325027, China; Department of Orthopedics, Qilu Hospital of Shandong University, Jinan, 250012, China.

|June 8, 2023

概括

循环螺旋B (CHBP) 通过促进自和减少编程细胞死亡,增强脊髓损伤 (SCI) 后的功能恢复. 这种可以作为SCI的新型治疗剂显示出希望.

科学领域:

神经科学是一个神经科学.
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

脊髓损伤 (SCI) 涉及被编程的细胞死亡途径,如死和热.
已知循环螺旋B (CHBP) 能维持红色素 (EPO) 的活性,并提供组织保护.
在SCI中CHBP的精确神经保护机制在很大程度上仍未被探索.

研究的目的:

调查CHBP在SCI中的神经保护作用背后的死和烧相关机制.
阐明自和特定信号通路在CHBP介导的SCI恢复中的作用.

主要方法:

利用基因表达综合 (GEO) 数据集和RNA测序来识别分子标.
为组织学和行为评估 (HE,Nissl,Masson染色,BMS,足迹分析) 建立了伤SCI的小鼠模型.
采用qPCR,西斑,免疫沉和免疫光来分析亡,烧亡,自和AMPK通路分子.

主要成果:

在SCI后,CHBP显著改善了功能恢复,增加了自,并抑制了亡和亡.
自抑制 (3-甲基氨酸) 降低了CHBP的有益作用.
通过AMPK-FOXO3a-SPK2-CARM1和AMPK-mTOR通路的TFEB脱和核转位来调解CHBP诱导的自升高.

结论:

CHBP有效调节自,减轻促炎性细胞死亡,并促进SCI后的功能恢复.
CHBP显示出作为治疗剂的潜力,用于治疗脊髓损伤的临床应用.

关键词:

AMPK信号通道的信号通道自自是一种自的过程.循环螺旋 B 的 B .有关炎症的细胞死亡.脊髓损伤导致的脊髓损伤

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