综合乳酸生产从热条件下的农业灰纤维素废弃物
Ya-Jun Liu1, Yuedong Zhang1, Fang Chi1
1CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, China; Shandong Energy Institute, Qingdao, China; Qingdao New Energy Shandong Laboratory, Qingdao, China; Dalian National Laboratory for Clean Energy, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China.
Journal of environmental management
|June 8, 2023
概括
这项研究开发了一种单发酵工艺,使用Geobacillus stearothermophilus 2H-3从农业废物中生产乳酸 (LA). 这种可持续的方法有效地将纤维素生物质转化为高纯度的LA.
科学领域:
- 生物技术和工业微生物学
- 可持续化学 可持续化学
- 生物精炼是生物精炼的方法之一.
背景情况:
- 对可生物降解的聚乳酸 (PLA) 的需求不断增长,需要可持续的生产方法.
- 农业废弃物为生物加工提供了丰富的基纤维素原料.
研究的目的:
- 开发一个高效的,综合的过程,以生产乳酸 (LA) 从农业灰纤维素废物.
- 为了利用热友菌株,Geobacillus stearothermophilus 2H-3,进行强大的LA生物合成.
主要方法:
- 隔离和表征的地球菌热 2H-3.
- 开发一个一个的连续发酵,整合全细胞合并生物糖化 (CBS) 和直接发酵.
- 来自玉米,玉米残留物和小麦的CBS水解剂的发酵.
主要成果:
- 在60°C和pH值6.5.5确定了最佳发酵条件.
- 成功地将G. stearothermophilus 2H-3直接注射到非消毒的CBS水解剂中.
- 获得高光学纯度 (99.5%),标位 (51.36 g/L) 和产量 (0.74 g/g生物质) 的L-(+) LA.
结论:
- 集成的CBS和G. stearothermophilus 2H-3发酵为可持续的LA生产提供了一个有希望的战略.
- 单工艺通过消除中间灭菌和调整来简化操作.
- 这种方法有效地将农业废物转化为高价值的乳酸.
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