OSCA-finder:通过代谢学和深度学习重新定义病诊断的测量方法
Xuanlin Meng1, Zhian He1, Lizhen Guo2
1Instrumental Analysis Center, Shanghai Jiao Tong University, Shanghai, 200240, China.
Talanta
|June 8, 2023
概括
尿道沟阻塞 (UPJO) 尿液分析通过OSCA-Finder管道得到了改进. 这种方法使用透压校准来准确发现生物标志物,增强UPJO患者的诊断.
科学领域:
- 生物化学 生物化学
- 分析化学 分析化学
- 生物标志物发现发现
背景情况:
- 液体染色体质谱法 (LC-MS) 对于尿液和血液分析至关重要.
- 尿样中的高变异性挑战了代谢物识别和生物标志物发现.
- 尿道关阻塞 (UPJO) 患者尿液中的肌氨酸度变化会影响标准校准策略.
研究的目的:
- 开发一种新的管道,OSCA-Finder,用于强大的尿液生物标志物分析.
- 提高尿样中代谢物识别的准确性和可靠性.
- 为UPJO患者建立可靠的诊断策略,使用尿液生物标志物.
主要方法:
- 提出了OSCA-Finder管道,将透压力和注射体积集成到校准中.
- 使用在线混合器稀释,以获得稳定的峰值形状和总离子色谱.
- 在神经网络二元分类器培训中采用了数据增强策略.
- 识别了具有峰值面积组变化系数 (CV) <30%的代谢物.
主要成果:
- 实现了神经网络二进制分类器准确率为99.9%.
- 成功识别了更多的代谢物,并获得了更多的每样尿液峰值.
- 开发了一种诊断策略,使用七个生物标志物将UPJO患者与健康个体区分开来.
- 证明了尿液奥斯莫斯压力校准在标准方法上的潜力.
结论:
- 在OSCA-Finder管道显著提高尿液生物标志物分析的准确性.
- 基于透压的校准为UPJO生物标志物发现提供了更可靠的方法.
- 开发的诊断策略显示了UPJO诊断中临床应用的巨大潜力.
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