细胞外细胞染色体纳米线似乎在 prokaryotes 中无处不在
Diana P Baquero1, Virginija Cvirkaite-Krupovic1, Shengen Shawn Hu2
1Institut Pasteur, Université Paris Cité, CNRS UMR6047, Archaeal Virology Unit, Paris 75015, France.
Cell
|June 8, 2023
概括
现在在古生物中发现了细胞外纳米线 (ECN),而不仅仅是细菌. 这些导电蛋白丝对于电子转移至关重要, 似乎是一种广泛的生物机制.
科学领域:
- 微生物学
- 生物化学
- 结构生物学
背景情况:
- 来自Geobacter sulfurreducens的电导附属物,称为细胞外细胞纳米线 (ECN),已经引起了潜在应用的重大兴趣.
- 在细菌以外的生物体中,类似电子转移的ECN的流行程度在很大程度上是未知的.
研究的目的:
- 研究古生物中的细胞外纳米线 (ECN) 的存在和结构.
- 确定ECN是否是一种广泛的电子转移机制.
主要方法:
- 使用冷电子显微镜来确定超热友古生物的ECN原子结构.
- 生物信息分析用于识别各种古生物和等离子体中的ECN同类.
主要成果:
- 来自高热性古生物的两个不同的ECN的原子结构得到了阐明.
- 在中性甲氧化和降解的古生物,以及博格大质粒中发现了Archaeoglobus veneficus ECN的同类物.
- 尽管蛋白质折叠不同,但已识别的ECN具有保留的血排列,表明电子转移的优化包装.
结论:
- 在古生物中发现ECN表明这些导电蛋白丝是长距离电子转移的常见和广泛的机制.
- 这一发现将已知的细胞外电子转移的生物学作用扩展到两种 prokaryotic 领域:细菌和古生物.
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