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一种基于粘附的方法,用于快速且低成本地分离循环中的瘤细胞

Xinyi Ye¹, Jianjun Zou², Jing Chen¹

¹Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.

Clinica chimica acta; international journal of clinical chemistry

|June 8, 2023

概括

一种新的,低成本的方法快速隔离血液中的循环瘤细胞 (CTCs),利用它们的粘合性. 这种技术在各种癌症中实现了高捕获率,保持了下游分析的细胞活力.

科学领域:

生物技术是生物技术.
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

通过循环瘤细胞 (CTC) 的非侵入性癌症监测受到当前测试技术的限制.
从血液中有效,快速和经济有效地分离CTC,对于精确的癌症检测至关重要.

研究的目的:

开发一种新的,敏感的方法来隔离CTC.
为了实现癌症诊断的快速和经济有效的CTC丰富.

主要方法:

与白细胞相比,利用了CTCs的差异性粘合性.
采用牛血清白蛋白 (BSA) 涂层的微板和低速离心法进行细胞分离.
开发了一种用于CTC隔离的无标签工艺.

主要成果:

在各种癌症细胞系 (乳腺,肺,肝脏,宫,结直肠) 实现了高CTC捕获比率 (70.7-86.6%),具有不同的表型和大小.
证明了该方法在胰腺癌CTC检测方面的潜力.
保持高细胞活力 (∼99%),适合下游DNA/RNA测序.

结论:

一种新的,非破坏性的,快速的CTC缩技术已经成功开发出来.
从患者的血液和膜输液样本中成功分离了罕见的瘤细胞.
该方法在癌症诊断中的临床转化方面显示出显著的前景.

关键词:

粘合剂的差异是因为粘合剂的差异.循环中的瘤细胞.低成本和快速检测可以实现.

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