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在Karaoun湖使用LC-HRMS检测二次蓝藻细菌代谢物

Sevasti-Kiriaki Zervou¹, Noura Alice Hammoud², Simon Godin³

¹Laboratory of Photo-Catalytic Processes and Environmental Chemistry, Institute of Nanoscience & Nanotechnology, National Center for Scientific Research "Demokritos", Patr. Gregoriou E & 27 Neapoleos Str, 15431 Agia Paraskevi, Athens, Greece.

The Science of the total environment

|June 8, 2023

概括

有害的藻类开花会释放出毒素. 一种新的LC-HRMS方法在黎巴嫩卡拉昂湖中检测到92种代谢物,包括7种新型化合物,这凸显了对更好的检测工具的需求.

科学领域:

环境化学环境化学
生态毒理学生态毒理学
分析化学分析化学

背景情况:

由于气候变化和营养污染,全球有害藻类繁殖 (HAB) 正在增加.
菌在开花期间释放出强大的毒素和其他生物活性代谢产物.
这些化合物对水生生态系统和公共健康构成风险,需要强有力的检测方法.

研究的目的:

开发和应用液态染色学高分辨率质谱法 (LC-HRMS) 方法,用于检测环境样本中的蓝藻细菌代谢物.
为了调查卡拉昂湖,黎巴嫩的花样中的代谢物的多样性和身份.
为了确定新型的蓝菌代谢物,并评估人为污染物的存在.

主要方法:

开发一种LC-HRMS方法用于甲基分析.
使用复合物发现器软件和CyanoMetDB数据库进行数据处理.
分析了黎巴嫩卡拉昂湖 (Lake Karaoun) 的花样.

主要成果:

标注了92种代谢物,包括51种毒素 (微囊素,微素,气素等). ) 的情况.
发现了7种新的蓝色细菌代谢物:MC-ClYR,[epoxyAdda5]MC-YR,MC-LI,阿鲁吉诺辛638,阿鲁吉诺辛588,麦克罗吉宁755C和麦克罗吉宁727.
检测到人为污染物,表明湖泊污染和各种危险化合物的同时存在.

结论:

开发的LC-HRMS方法在环境样本中有效检测各种蓝藻细菌代谢物.
该研究确定了大量已知和新型的代谢物,强调了化学的复杂性.
强调对光谱图书馆和参考标准的关键需求,以准确识别和评估毒素和其他代谢物的风险.

关键词:

在CyanoMetDB中,使用的是CyanoMetDB.化是一种化.蓝色毒素 (Cyanotoxins) 是一种有毒物质.对LC-HRMS进行分析.微囊是一种微囊.二次的蓝藻细菌代谢产物.

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