在部分脱过程中,酸盐积累对C/NO-3-N比率升高的反应:关于细胞外聚合物质,微生物群落和代谢功能的洞察
Zilong Hou1, Wenyi Dong2, Hongjie Wang2
1School of Civil and Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China.
Bioresource technology
|June 8, 2023
概括
提高化学氧气需求与酸比率 (C/N) 影响部分脱 (PD). 最佳的C/N比率通过影响微生物群落和细胞外聚合物质来增强酸盐的积累.
科学领域:
- 环境微生物学环境微生物学
- 污水处理 污水处理 污水处理
- 生物地质化学循环是什么
背景情况:
- 部分脱 (PD) 对于废水处理中去除至关重要.
- 碳与的比率 (COD/NO3--N,C/N) 显著影响PD效率.
- 了解驱动酸盐积累的微生物和化学因素是必不可少的.
研究的目的:
- 研究C/N比率升高对PD期间酸盐积累的影响.
- 阐明细胞外聚合物质 (EPS) 和微生物群落的作用.
- 确定关键的微生物和酶体参与酸盐动态的参与者.
主要方法:
- 在PD系统中对C/N比率的实验操纵.
- 在紧密结合的EPS (TB-EPS) 中量化酸盐,多糖 (PS) 和蛋白质 (PN).
- 使用Illumina MiSeq测序进行微生物社区分析.
- 对酸盐减少酶 (narG/H/I) 和酸盐减少酶 (nirK) 的酶活性测定.
- 冗余分析 (RDA) 用于关联环境和微生物因素.
主要成果:
- 化物在C/N比率1.53.0积累和稳定,在更高比率 (4.05.0) 降低.
- 在TB-EPS中的多糖和蛋白质含量在C/N2.53.0的峰值,可能受到酸盐的刺激.
- 图埃拉和OLB8是低C/N的占主导地位的属,而图埃拉的丰富度是高C/N的.
- 高的Thauera丰度与酸盐减少酶 (nirK) 活性增加相关.
- 在低C/N时,RDA显示了酸盐产量,PN含量,特定细菌 (Thauera,OLB8) 和酸盐减少酶 (narG/H/I) 之间的正相关性.
结论:
- 在部分脱过程中,C/N比率是控制酸盐积累的关键因素.
- 微生物社区结构 (Thauera丰富) 和TB-EPS组成 (PN) 是关键的调解者.
- 微生物种群,EPS和酶性活动之间的协同相互作用推动了酸盐的积累.
关键词:
COD/NO3 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -亚酸盐的积累 亚酸盐的积累亚酸盐还原酶是一种酸盐.OLB8 属 属 属 属冗余性的分析分析.更多相关视频
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