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相关概念视频

In-vitro Mutagenesis01:16

In-vitro Mutagenesis

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To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
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相关实验视频

Updated: Jul 27, 2025

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
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在基因组规模的向突变发生中.

Jianjie He1,2, Kai Zhang1,2, Shuxiang Yan1,2

  • 1Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

Genome research
|June 8, 2023
PubMed
概括

这项研究引入了一个聚合的CRISPR库,用于有效的基因组规模的针对性编辑,用于allotetraploid作物Brassica napus. 这种方法推进了功能性基因组学和多体植物中的作物育种.

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Optogenetic Random Mutagenesis Using Histone-miniSOG in C. elegans
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Last Updated: Jul 27, 2025

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
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科学领域:

  • 植物基因组学 植物基因组学
  • 生物技术是生物技术.
  • 农作物科学 农作物科学

背景情况:

  • 克里斯普尔-Cas9技术使得双胞胎作物中的大规模功能基因组学成为可能.
  • 在复杂的多倍体基因组中,有针对性的突变发生仍然是一个重大挑战.

研究的目的:

  • 为了证明一个聚合的CRISPR库的可行性,用于基因组规模的针对性编辑在allotetraploidBrassica napus.
  • 建立一种高通量方法,用于在多体植物中进行逆基因查.

主要方法:

  • 设计了18414个sgRNA,针对布拉西卡纳普斯的10480个基因.
  • 产生了1104种转基因植物,并分析了基因编辑效率.
  • 调查了目标地点的Cas9裂纹模式.

主要成果:

  • 在178个分析的基因中实现了52.2%的突变率.
  • 发现Cas9在多倍体中通过单个sgRNA引导切割所有目标部位.
  • 确定了影响脂肪酸特征和种子油含量的新型基因.

结论:

  • 聚合的CRISPR图书馆是有效的高通量向突变发生在allotetraploid作物.
  • 这种方法为Brassica napus功能基因组学和育种提供了宝贵的资源.
  • 为多体植物研究和作物改良提供参考资料.