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Updated: Jul 27, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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模板跳跃主要编辑允许大插入和exon重写 in vivo

Chunwei Zheng1, Bin Liu1, Xiaolong Dong1

  • 1RNA Therapeutics Institute, University of Massachusetts Chan Medical School, Worcester, MA, 01605, USA.

Nature communications
|June 8, 2023
PubMed
概括

模板跳转初始编辑 (TJ-PE) 允许精确插入高达500bp的大型DNA片段. 这种新型的基因组工程工具成功地在体内重写外因子以逆转疾病表型,为基因治疗提供了一种新方法.

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