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CD5阳性毛细胞白血病的流动细胞计

Diana Cenariu¹, Ioana Rus², Jon Thor Bergthorsson³

¹Medfuture Research Center for Translational Medicine, Iuliu Hatieganu University of Medicine and Pharmacy, Louis Pasteur Street, Cluj-Napoca, Romania.

Molecular diagnosis & therapy

|June 8, 2023

概括

诊断具有非典型CD5表达的毛状细胞白血病 (HCL) 可能具有挑战性. 流细胞计 (FC) 对于准确的分类和及时的治疗启动至关重要,特别是当CD5标记物异常存在时.

科学领域:

血液学血液学血液学
在瘤学瘤学.
免疫型定型免疫型定型

背景情况:

毛细胞白血病 (HCL) 是一种慢性淋巴增殖性疾病.
诊断通常是直接使用骨髓形态和流细胞计 (FC) 或免疫组织化学.
细胞表面标记物的非典型表达,如CD5,可以使差异诊断复杂化.

研究的目的:

描述HCL与非典型的CD5表达的诊断.
强调FC在诊断这些具有挑战性的病例中的作用.
从其他淋巴增殖性疾病中区分具有非典型CD5的HCL.

主要方法:

对具有非典型CD5表达的HCL进行详细的诊断方法.
与相似的淋巴增殖性疾病的差异诊断.
骨髓吸附物的FC分析,包括关门策略和标记物表达 (CD45,CD19,CD25,CD11c,CD20,CD103,CD10,CD3,CD4,CD8,CD5).

主要成果:

FC分析确定了具有非典型CD5表达的HCL的特定标记者配置文件.
细胞对CD25,CD11c,CD20,CD103呈阳性,并显示出CD5的明亮表达.
CD10是暗变为负的,T细胞标记物 (CD3,CD4,CD8) 也是正的.

结论:

在HCL中非典型的CD5表达使诊断复杂化,但可以通过FC进行管理.
FC使HCL的最佳分类成为可能,即使具有不寻常的标记物呈现.
根据准确的FC诊断,可以及时和令人满意的治疗,如克拉迪宾化疗,可以开始.

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相关概念视频

Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...