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Meng-Jie Zhou¹, Jing Wu¹, Liu-Xiu Hu^1,2

¹College of Biological and Food Engineering, Anhui Polytechnic University, Wuhu, 241000, China.

World journal of microbiology & biotechnology

|June 8, 2023

概括

添加像Tween-80这样的表面活性剂显著增加了menaquinone-7 (MK-7) 的产生,这是必不可少的维生素K2营养素. 这种优化增强了Bacillus subtilis中MK-7的合成,为工业发酵的进步铺平了道路.

科学领域:

生物技术是生物技术.
微生物学微生物学
生物化学生化学

背景情况:

menaquinone-7 (MK-7),维生素K2的重要形式,在人类健康中起着至关重要的作用,包括骨和心血管健康.
目前的MK-7生产方法依赖于微生物发酵,目前的研究重点是优化产量.
细菌168 KO-SinR是一种用于MK-7合成的突变菌株.

研究的目的:

为了研究表面活性剂对MK-7由 Bacillus subtilis 168 KO-SinR.的代谢合成的影响.
用特定的表面活性剂确定增强MK-7生产的最佳条件.
了解表面活性剂影响MK-7生物合成的潜在机制.

主要方法:

使用扫描电子显微镜和流式细胞计量来分析细胞膜的透性和生物膜结构.
使用定量实时PCR来评估MK-7合成相关基因的表达.
进行不同度表面活性剂的发酵实验,特别是Tween-80.

主要成果:

表面活性剂的添加改变了Bacillus subtilis 168 KO-SinR.的细胞膜透性和生物膜结构.
包括0.7%的Tween-80导致细胞外和细胞内MK-7产量分别为28.8 mg/L和59.2 mg/L.
总的MK-7合成增加了80.3%,添加了0.7%的Tween-80,伴随着显著上调的基因表达.

结论:

表面活性剂,特别是Tween-80,有效地增强了Bacillus subtilis中MK-7的代谢合成.
该机制涉及细胞膜透率的增加和生物膜结构的调节,从而改善了MK-7的生产.
这些发现为工业规模的MK-7发酵和生产提供了宝贵的见解.

关键词:

这种细菌是 Bacillus subtilis.扫描电子显微镜扫描电子显微镜表面活性剂是什么?表面活性剂是什么?合成途径合成途径 qRT-PCR 是一个很好的方法.

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