相关实验视频

Updated: Jul 27, 2025

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Selected Reaction Monitoring Mass Spectrometry for Absolute Protein Quantification

Published on: August 17, 2015

通过UPLC-MS/MS对AT7519作为一种促进溶解的化合物的分析,在一种由乙氨基诱导的小鼠模型中,通过UPLC-MS/MS对急性炎症进行分析

Jennifer A Cartwright^1,2, Joanna P Simpson³, Natalie Z M Homer³

¹University of Edinburgh Centre for Inflammation Research, Institute for Regeneration and Repair, 4-5 Little France Drive, Edinburgh BioQuarter, Edinburgh, Midlothian, EH16 4UU, United Kingdom. Jennifer.Cartwright@ed.ac.uk.

Journal of inflammation (London, England)

|June 8, 2023

概括

我们开发了一种新的LC-MS/MS方法,用于在小鼠中测量AT7519和乙氨基 (APAP). 随着APAP诱导的肝损伤,AT7519水平增加,但没有恶化肝损伤.

科学领域:

药理学药理学是指药理学的学科.
毒理学毒理学毒理学
分析化学分析化学

背景情况:

失控的炎症会加剧器官损伤,例如乙氨基诱导的急性肝损伤 (APAP-ALI).
AT7519,一种循环素依赖性激酶抑制剂 (CDKI),可以解决炎症,但其在APAP-ALI中的作用尚未研究.
目前的方法缺乏在小鼠模型中同时测量APAP和AT7519的能力.

研究的目的:

开发和验证一种敏感的LC-MS/MS方法,用于在小鼠血清中量化AT7519和APAP.
研究AT7519对APAP代谢的影响及其对APAP-ALI的影响.
在APAP诱导的肝损伤的背景下,评估AT7519在10毫克/公斤剂量的安全性.

主要方法:

优化了一种使用电喷射电离的液体染色学-双重质谱法 (LC-MS/MS) 方法.
使用了Acquity UPLC BEH C18柱和水和甲醇的梯度移动相.
验证了该方法的线性,精度,准确性,并将其应用于小鼠剂后血清样本.

主要成果:

建立了一种优化,敏感的LC-MS/MS方法,用于在小鼠血清中同时量化AT7519和APAP.
血清AT7519度在APAP诱导的肝损伤的小鼠中明显高于对照组.
没有观察到APAP和AT7519水平之间的相关性,也没有观察到AT7519和肝损伤或扩散的标志物之间的相关性.

结论:

开发的LC-MS/MS方法在小鼠血清中准确量化AT7519和APAP,在APAP毒性的小鼠模型中证明有效.
在接受APAP治疗的小鼠中AT7519的升高表明CDKI的肝脏代谢.
在APAP-ALI中,AT7519 (10 mg/kg) 的测试剂量没有加剧肝损伤或阻碍修复机制.

关键词:

肝脏病肝脏病是一种肝脏病.质谱测量质量谱测量作为一个中性粒细胞.帕拉西他摩尔是一种可怕的药物.

更多相关视频

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

Author Spotlight: Evaluating Traditional Chinese Therapy for Ankylosing Spondylitis in Mice

Author Spotlight: Evaluating Traditional Chinese Therapy for Ankylosing Spondylitis in Mice

Published on: October 27, 2023

相关概念视频

¹H NMR Signal Integration: Overview

¹H NMR Signal Integration: Overview

The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...