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菌菌的COQ2基因编码了一个功能性的4-基酸聚烯转移酶

Camila Andrea Zafra¹, Marcell Crispim¹, Ignasi Bofill Verdaguer¹

¹Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, Avenida Professor Lineu Prestes, 1374, Butantã, 05008-000, São Paulo, Brazil.

FEMS microbiology letters

|June 8, 2023

概括

菌的PF3D7_0607500基因产物PfCOQ2作为4-基酸聚烯转移酶起作用,对于乌比奎农生物合成至关重要. 这项研究描述了它在Plasmodium ubiquinone生产中的作用.

科学领域:

生物化学生物化学
寄生虫学的寄生虫学
线粒体生物学线粒体生物学

背景情况:

乌比金 (UQ) 对线粒体电子运输链至关重要.
UQ生物合成涉及4-基酸聚烯转移酶 (EC 2.5.1.39).
这种酶在Plasmodium物种中没有特征.

研究的目的:

为了描述Plasmodium falciparum PF3D7_0607500 (PfCOQ2) 的功能.
要确定PfCOQ2是否编码一个功能性的4-基酸聚烯转移酶.
为了研究在Plasmodium中的ubiquinone生物合成前体.

主要方法:

在一个Saccharomyces cerevisiae coq2Δ突变体中表达的Plasmodium falciparum PF3D7_0607500.
在糖介质上评估了酵母生长缺陷的补充.
在脂质提取物中使用放射性标记前体的代谢标记识别了ubiquinone.

主要成果:

PfCOQ2表达补充了酵母生长缺陷.
在表达PfCOQ2的酵母中检测到乌比金,不管是用p-aminobenzoic酸或4-hydroxybenzoic酸标记.
在Plasmodium falciparum中,p-aminobenzoic酸并没有作为ubiquinone的前体.

结论:

PfCOQ2是Plasmodium falciparum中的一个功能性的4-基酸聚烯转移酶.
在Plasmodium中,pfCOQ2使用4-基酸,但不使用p-aminobenzoic酸作为前体.
由于p-aminobenzoic酸不能作为前体,这可能是由于上游监管机制.

关键词:

帕拉-阿米诺子酸是什么?诺基诺是什么诺基诺是什么异种表达的异种表达.疟疾疟疾是一种疾病.呼吸系统呼吸系统呼吸系统这种药物是Ubiquinone.

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