综合转录组和代谢组分析揭示了葡萄树中暴露于盐和应激的抗氧化机制
Xu Lu1, Guiping Chen2, Lei Ma2
1College of Horticulture, Gansu Agricultural University, Lanzhou, People's Republic of China.
Physiologia plantarum
|June 9, 2023
概括
葡萄树表现出明显的抗氧化防御盐和压力,压力导致更高的氧化损伤. 黄类生物合成是盐应激适应的关键.
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 植物适应盐和等非生物应激依赖于抗氧化系统来管理活性氧物种 (ROS).
- 了解差异性应激反应对于作物改善至关重要.
研究的目的:
- 研究盐和应激对葡萄藤生理学的影响,重点关注氧化应激标记物,抗氧化酶活动,基因表达和代谢物概况.
- 阐明葡萄树在盐与压力下采用的独特适应机制.
主要方法:
- 在盐和压力下对反应性氧物种 (ROS),和电解质泄漏的比较分析.
- 酶活性测定关键的抗氧化酶.
- 转录组和代谢组分析,以确定分子和代谢变化.
- 转录组和代谢组数据的综合分析.
主要成果:
- 盐和应激都增加了ROS,甲和电解质泄漏,应激诱导了更高的水平.
- 抗氧化酶活动显示了对压力的各种反应.
- 转录组分析揭示了与信号转导和抗氧化剂通路相关的差异性基因表达.
- 代谢分析表明,在盐应激下,酸和谷氨的含量增加,在两种应激下,酸,黄酸和酸的含量增加.
- 黄类生物合成被确定为对盐应激反应的关键途径,与应激相比,在盐应激下黄类积累更高.
结论:
- 葡萄树在盐和的压力下显示出明显不同的抗氧化剂防御策略.
- 性压力比盐性压力更严重的氧化负担.
- 黄酸积累是葡萄酒适应盐应激的一个关键组成部分.
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