变形性和碰撞引起的重定向增强了密集的微环境中的细胞拓作用
Leonie van Steijn1, Joeri A J Wondergem2, Koen Schakenraad3
1Mathematical Institute, Leiden University, Leiden, the Netherlands.
Biophysical journal
|June 9, 2023
概括
细胞的变形性和复杂的相互作用增强了topotaxis,沿着障碍物密度梯度的导航. 新的模型显示细胞形状和支柱相互作用显著影响细胞向较低密度移动.
科学领域:
- 细胞动力学和生物物理学
- 计算机生物学和建模
背景情况:
- 细胞在复杂的环境中使用诸如障碍密度梯度等线索进行导航,这种现象称为topotaxis.
- 之前的活跃布朗粒子 (ABP) 模型预测了比实验观察到的更低的顶点动态漂移.
研究的目的:
- 通过结合细胞变形性和复杂的细胞柱相互作用来调查拓西斯预测中的差异.
- 开发和验证在地形环境中用于细胞导航的更详细模型.
主要方法:
- 利用细胞波茨模型 (CPM) 与Act模型 (模仿actin驱动的机动性) 以及混合CPM-ABP模型.
- 调整模型参数以实验Dictyostelium discoideum在平面上的运动.
- 在柱状网格中模拟托帕克斯,柱子密度不同.
主要成果:
- CPM变体预测的顶点动态漂移比ABPs更高,更好地与饥饿的Dictyostelium discoideum的实验数据保持一致.
- 在密集的柱子网中,ACT模型表现出卓越的拓效率,这是由于在密集的柱子网中,持久时间的减少更大.
- 支柱粘附减少了细胞速度和拓作用;较不持久的植物细胞显示最小漂移.
结论:
- 与ABPs相比,可变形细胞体积增加了顶点动力漂移.
- 细胞柱碰撞对细胞持久性的反会增加漂移,特别是在高度持久的细胞中.
- 基于CPM的模型在复杂的环境中提供了更准确的细胞拓作用的表现.
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