基于Rab7/Retromer的内分泌体贩运对于在水爆发中进行适当的宿主入侵至关重要
Xin Chen1,2, Poonguzhali Selvaraj3, Lili Lin1,4
1State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
The New phytologist
|June 9, 2023
概括
这项研究揭示了大米爆发真菌Magnaporthe oryzae中一种新的途径,其中MoRab7和逆转基因复合体通过内分泌体介导效应因子分泌. 这种机制对于真菌病原性和宿主-病原体相互作用至关重要.
科学领域:
- 植物病理学 植物病理学
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
背景情况:
- 植物病原体分泌作用因子,抑制宿主免疫力并促进感染.
- 了解效应物分泌机制是控制植物疾病的关键.
研究的目的:
- 调查Magnaporthe oryzae中的新型膜贩运和传递途径的效应体分泌物.
- 阐明MoRab7,逆转子和MoSnc1在这个过程中的作用.
主要方法:
- 囊泡贩运的活细胞成像.
- 对蛋白质与蛋白质相互作用的分析 (MoRab7,复原体,MoSnc1).
- 确定的途径的遗传破坏.
主要成果:
- 确定了一条从真空膜发起到宿主接口/血膜的新途径.
- 莫拉布7调用逆转激素复合体来调解像MoSnc1.1这样的SNARE蛋白的识别和贩运.
- 这种途径的干扰会损害效应物分泌和真菌病原性.
结论:
- 一种非传统的内溶性体分泌路径对于Magnaporthe oryzae的致病性至关重要.
- 在米爆病期间,MoRab7/Retromer/MoSnc1机械在效应体分泌中起着至关重要的作用.
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