相关实验视频

Updated: Aug 10, 2026

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Three-Dimensionally Printed Microfluidic Cross-flow System for Ultrafiltration/Nanofiltration Membrane Performance Testing

Published on: February 13, 2016

3D打印的集成纳米孔膜用于DNA的电提取

Hari Kalathil Balakrishnan^1,2, Soo Min Lee¹, Ludovic F Dumée^3,4

¹Deakin University, Centre for Rural and Regional Futures, Locked Bag 20000, Geelong, VIC 3320, Australia. rosanne.guijt@deakin.edu.au.

|June 9, 2023

概括

研究人员开发了一种3D打印方法,以创建用于DNA提取设备的纳米孔膜. 这种3D打印的尺寸移动性陷简化了在需要时进行核酸放大测试.

科学领域:

材料科学材料科学材料科学
生物技术是生物技术.
微流体学微流体学

背景情况:

3D打印提供了微型制造能力,但直接打印亚微孔是有限的.
纳米孔状材料可以集成到3D打印设备中,以创建功能性孔状特征.
聚合诱导相分离 (PIPS) 是一种用于制造纳米多孔材料的方法.

研究的目的:

开发一种3D打印方法,用于制造具有受控孔径的纳米孔隙膜.
将这些膜整合到一个功能性流体装置中,用于DNA提取.
评估用于DNA净化和检测的3D打印设备的性能.

主要方法:

数字光投影 (DLP) 3D打印与PIPS树脂配方一起使用.
树脂交换被用来创建一个功能性流体装置与集成的纳米孔状膜.
暴露时间,光启动剂度和孔子含量各不相同,以控制孔子大小 (30-800 nm).
一个尺寸移动性陷是用346nm和30nm孔径的膜制造的.

主要成果:

成功制造出3D打印的纳米孔膜,可调节的孔径为30-800nm.
使用树脂交换DLP3D打印创建了一个功能性尺寸移动性陷设备.
该装置在qPCR后检测到低细胞度 (10^3细胞/毫升) 的DNA.
陷有效地净化了DNA,从溶解酸中去除了73%的蛋白质.
DNA提取产量与旋转柱相当,但手动处理和设备需求显著减少.

结论:

量身定制的纳米孔隙膜可以使用简单的树脂交换DLP制造工艺集成到流体设备中.
开发的尺寸移动性陷可方便从复杂的溶解物中提取和净化DNA.
这种方法可以减少DNA提取的处理时间,手动处理和设备要求.
该技术显示出在需要点诊断核酸放大测试的潜力,因为其可制造性,便携性和易用性.

更多相关视频

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Creating Sub-50 Nm Nanofluidic Junctions in PDMS Microfluidic Chip via Self-Assembly Process of Colloidal Particles

Published on: March 13, 2016

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

相关概念视频

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...