Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Exploring the influence of internal surface modification of paramagnetic mesoporous silica nanoparticles on MRI relaxation dynamics.

Journal of materials chemistry. B·2026
Same author

Unraveling Quinone Degradation Enables Stabilization Using Redox Helpers in Biological and Electrochemical Systems.

Journal of the American Chemical Society·2026
Same author

Post-catalysis structures of mitochondrial complex I with ubiquinol-10 bound in the active site.

Nature communications·2026
Same author

Electron hopping in conjugated molecular wires with application to solar cells.

Nature chemistry·2026
Same author

Light-Activated Metal-Dependent Protein Degradation: A Heterobifunctional Ruthenium(II) Photosensitizer Targeting New Delhi Metallo-β-lactamase 1.

Journal of the American Chemical Society·2025
Same author

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Science advances·2025

相关实验视频

Updated: Jul 27, 2025

A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

14.0K

使用光散射来评估脂蛋白相互作用如何影响脂质体中的复合I功能.

Jana Eisermann1, John J Wright2, James D E T Wilton-Ely1

  • 1Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, White City Campus London W12 0BZ UK m.roessler@imperial.ac.uk j.wilton-ely@imperial.ac.uk.

RSC chemical biology
|June 9, 2023
PubMed
概括

电泳光散射 (ELS) 揭示了蛋白质体中泽塔电位变化与复合I功能相关. 心脏脂蛋白至关重要,作为膜蛋白活性和质子效率的敏感指标.

更多相关视频

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

8.0K
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.1K

相关实验视频

Last Updated: Jul 27, 2025

A Fluorescence-based Assay of Phospholipid Scramblase Activity
09:52

A Fluorescence-based Assay of Phospholipid Scramblase Activity

Published on: September 20, 2016

14.0K
A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
09:12

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes

Published on: December 13, 2019

8.0K
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
08:49

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

4.1K

科学领域:

  • 生物化学 生物化学
  • 生物物理学的生物物理.
  • 膜蛋白研究研究 膜蛋白研究

背景情况:

  • 复合物I对细胞呼吸至关重要,产生ATP合成的质子梯度.
  • 蛋白质体提供了一个简化的模型来研究复合物I活动和质子运输.
  • 了解膜蛋白重构和脂质双层中的功能至关重要.

研究的目的:

  • 调查物理参数,特别是泽塔潜力与蛋白质体中复合I的生物化学功能之间的相关性.
  • 评估心脏脂蛋白在复合物I复合物复合和活性中的作用.
  • 探索电泳光散射 (ELS) 作为评估基于膜蛋白质的蛋白质体的工具.

主要方法:

  • 采用了动态和电泳光散射 (DLS和ELS) 技术.
  • 复合物I被重组成具有不同脂质组成的脂质体.
  • 泽塔潜能测量与蛋白质保留和催化活性相关.

主要成果:

  • 泽塔潜力与蛋白质体中的复合I生化功能有很强的相关性.
  • 卡迪奥利平在复合物I的复合和功能中起着至关重要的作用,作为一个敏感的记者.
  • 泽塔电位的变化与蛋白质保留和氧化还原活性线性相关.
  • 泽塔电位变化对复合I产生的质子动力很敏感.

结论:

  • 泽塔潜力,特别是在心脏脂蛋白的存在下,是蛋白质体生物化学能力的可靠指标.
  • ELS测量为评估脂质系统中的膜蛋白活性提供了一种有价值的补充技术.
  • 这种方法促进了对膜蛋白的研究,特别是那些涉及带电脂和质子运输的膜蛋白.