相关实验视频

Updated: Jul 26, 2026

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

一个简化的策略,用于商业积极选择载体的自我生产,即pJET1.2/blunt克隆载体,使用常见的实验室

Omar Nawawi¹, Mohd Puad Abdullah², Chong Yu Lok Yusuf^1,3

¹Laboratory of Plant Genetic and Cell Biology, Faculty of Plantation and Agrotechnology, Universiti Teknologi MARA, Jasin Campus, 77300 Merlimau, Melaka Malaysia.

|June 9, 2023

概括

一种新方法简化了pJET1.2/blunt克隆载体的生产,消除了凝净化. 这种更快,更具成本效益的DNA克隆策略使用常见的大肠杆菌菌株产生超过98%的阳性克隆.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
基因工程是一种基因工程.

背景情况:

积极选择向量对于分子克隆至关重要,利用致命的基因来确保仅保留所需结构.
之前的pJET1.2/blunt克隆载体的内部生产方法需要广泛的凝电泳和净化步骤.
这些净化步骤耗时,增加了DNA克隆程序的总体成本.

关键词:

在DNA克隆过程中,积极的选择选择是积极的选择.pJET1.2/粗的克隆载体 pJET1.2N等离子体

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Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Published on: January 28, 2016

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

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