在SARS-CoV-2受体结合区域使用单克隆抗体识别保存的线性表位
Yujie Yang1, Liling Zhou1, Chuncong Mo1
1State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, 510182, China.
Heliyon
|June 9, 2023
概括
研究人员在SARS-CoV-2尖端蛋白上发现了三种新的线性表位.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 由SARS-CoV-2引起的COVID-19流行病构成了全球健康挑战.
- SARS-CoV-2 尖峰 (S) 蛋白,特别是它的受体结合域 (RBD),是疫苗和治疗的关键目标.
- 在RBD上的线性表位对疫苗开发至关重要,但尚未得到充分探索.
研究的目的:
- 针对SARS-CoV-2 S1蛋白的单克隆抗体 (mAbs) 的特征.
- 在RBD上识别新的线性表征.
- 评估突变对抗体识别和中和活性的影响.
主要方法:
- 针对SARS-CoV-2 S1蛋白的151只小鼠单克隆抗体的表征.
- 使用真核 SARS-CoV-2 RBD 和 Omicron 变异 S 蛋白质进行表皮图谱绘制.
- 伪病毒中和试验. 伪病毒中和试验.
- 对突变S蛋白的抗体反应性的分析 (N501Y,E484K,D614G).
主要成果:
- 在RBD (R6,R12,R16) 上确定了三个新的,保存的线性表位.
- 69个mAbs与Omicron变种发生反应,这表明在快速诊断中具有实用性.
- 一些已识别的mAbs,包括一个针对R12的mAbs,显示出对SARS-CoV-2的中和活性.
- 单个氨基酸突变显著影响了mAb识别和S蛋白结构.
结论:
- 已识别的线性表位在SARS-CoV-2变体中保存,并且可以在患者血清中检测到.
- 这些表位和中和抗体为开发诊断和治疗药物提供了潜力.
- 了解突变对抗体结合的影响对于有效的COVID-19对策开发至关重要.
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