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相关概念视频

Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
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The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
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Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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基因组测序的基因组测序

Nashwa Tarek1,2, Ahmed F Azmy3, Ahmed S Khairalla3,4

  • 1Department of Botany and Microbiology, Faculty of Science, Beni-Suef University, Egypt.

Heliyon
|June 9, 2023
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概括

从健康的口腔中分离出来的Enterococcus faecium NT04显示出益生菌的潜力. 基因组分析证实了抗微生物化合物的基因,支持其用于对抗口腔病原体.

关键词:
肠球菌 (Enterococcus faecium) 是一个有毒的菌株.在口腔腔中,口腔腔腔.益生菌 益生菌 益生菌在WGS中,使用的是WGS.

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科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.
  • 益生菌 益生菌 益生菌

背景情况:

  • 口腔微生物组在健康和疾病中起着至关重要的作用.
  • 识别具有益生菌性质的有益细菌对于口腔健康至关重要.
  • 肠球菌 (Enterococcus faecium) 在人类口腔中发现,但其益生菌潜力需要进一步研究.

研究的目的:

  • 从健康的成年人口腔中分离和描述一种新型益生菌细菌.
  • 为了研究隔离菌株益生菌和抗生素性质的基因组基础.
  • 评估这种分离物作为抗口腔病原体益生菌候选物的潜力.

主要方法:

  • 使用16S rRNA基因测序对口腔细菌进行隔离和鉴定.
  • 鉴定出的菌株 (Enterococcus faecium NT04) 的全基因组测序.
  • 生物信息分析用于基因注释,代谢途径的识别和毒性因子.

主要成果:

  • 这种细菌被鉴定为Enterococcus faecium NT04.
  • 基因组分析揭示了编码细菌素类抑制物质 (素A和B),抗氧化剂和维生素的基因.
  • 该基因组缺乏致病性岛屿和等离子体插入,表明宿主殖民而不是入侵毒性.

结论:

  • 菌 (Enterococcus faecium NT04) 具有显著的益生菌和抗菌特性.
  • 基因组概况支持该菌株作为一种安全有效的益生菌剂对抗口腔病原体的潜力.
  • 进一步的研究可以探索其在口腔保健产品中的应用.