没有等离子体的作弊细胞通常在实验室生长过程中进化
Amber M Bedore1, Christopher M Waters1
1Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA, 48824.
bioRxiv : the preprint server for biology
|June 9, 2023
概括
塑体中的抗生素耐药性基因可以在细菌生长过程中丢失,特别是在表面. 这种等离子体损失,即使是抗生素选择,也会产生混合细胞种群,并可能混实验.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 抗生素对于临床使用和实验室等离子体选择至关重要.
- 抗生素耐药性机制可以作为公共产品,使邻近细胞受益.
- 这些合作机制对实验室环境中的等离子体稳定性的影响尚不清楚.
结论:
- 抗生素耐药性机制可能导致实验室环境中意外的等离子体损失.
- 这种等离子体损失产生了耐药和易受细胞的混合种群,使实验结果复杂化.
- 研究人员必须考虑等离子体稳定性,当使用抗生素选择等离子体维护.
相关概念视频
01:28Plasmids
55
Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
55
01:16Viral Replication: Lysogenic Cycle
87
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
87
00:57Antibiotic Selection
54.8K
Overview
54.8K