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Updated: Jul 27, 2025

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture
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Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

Published on: August 8, 2013

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通过RNA-seq的进步改善RNA动态的研究,以代谢标记和核酸记录化学

Joshua T Zimmer1,2, Isaac W Vock1,2, Jeremy A Schofield1,2,3

  • 1Department of Molecular Biophysics & Biochemistry, Yale University, New Haven, CT 06511, USA.

bioRxiv : the preprint server for biology
|June 9, 2023
PubMed
概括

用4-thiouridine (s4U) 进行RNA代谢标记可能会因中断而丢失数据. 优化的协议和计算管道显著提高了RNA测序实验的准确性.

关键词:
4 - 氨基氨酸是什么?核酸记录化学的核酸记录化学在RNA-seqqq.斯拉姆斯兰姆斯兰姆斯兰姆斯兰姆斯兰姆斯兰姆斯兰姆斯兰姆斯兰姆斯在TUC中,你会看到TUC.时间的流逝,时间的流逝

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Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil Ers4tU00:11

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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