在蛋白质分泌到金属酸盐外酶过程中TerC蛋白的功能
Bixi He1, Ankita J Sachla1, John D Helmann1
1Department of Microbiology, Cornell University, 370 Wing Hall, 123 Wing Drive, Ithaca, New York 14853-8101, USA.
Research square
|June 9, 2023
概括
像MeeF和MeeY这样的TerC家族蛋白质对于金属化出口酶,特别是依赖的酶至关重要,确保了蛋白质的适当功能. 它们的缺失会损害蛋白质出口和分泌蛋白质中的含量.
科学领域:
- 微生物学 微生物学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 细胞质金属酶通过细胞内获取金属,但对出口酶的金属化机制的理解较少.
- 适当的化对于许多分泌和膜相关酶的功能至关重要.
研究的目的:
- 调查TerC家族蛋白质在出口酶的化中的作用.
- 阐明通过细胞外酶获得 (Mn) 的机制.
- 了解MeeF和MeeY在蛋白质分泌和酶活性中的功能.
主要方法:
- 研究的细菌细菌菌株缺乏MeeF (YceF) 和MeeY (YkoY).
- 分析了分泌蛋白质中的蛋白质出口能力和含量.
- 与一般分泌通路蛋白进行了配净试验.
- 评估了依赖Mn2+的脂肪酸合成酶 (LtaS) 的功能.
主要成果:
- 缺乏MeeF和MeeY的菌株显示蛋白质出口减少,分泌蛋白质中含量明显降低.
- 发现MeeF和MeeY与总分泌路径组件共净化.
- 缺少MeeF和MeeY使得FtsH膜蛋白酶对生存能力至关重要.
- MeeF和MeeY对于依赖Mn2+的脂铁酸合成酶 (LtaS) 的有效功能至关重要.
结论:
- MeeF和MeeY是保存的TerC家族的成员,它们通过一般分泌途径在酶出口过程中促进了酶的协同转位化.
- 这些蛋白质对于正确的 (Mn) 纳入膜结合和细胞外酶至关重要,确保它们的功能活性.
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