在没有新生组合的情况下,中粒体特异性核细胞在老化的小鼠卵细胞中持续存在
Arunika Das1,2, Katelyn G Boese1, Kikue Tachibana3
1Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USA.
bioRxiv : the preprint server for biology
|June 9, 2023
概括
在雌性哺乳动物中,在长细胞周期停止期间,在没有新的CENP-A组件的情况下,保持了中间体标识. 这挑战了已建立的中间体传播和遗传模型.
科学领域:
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 中介质对遗传遗传至关重要,但由CENP-A在表观遗传上定义.
- 已建立的模型表明,细胞周期合复制和G1受限制的组合保持了中位素的同一性.
- 在雌性哺乳动物中,延长的预相I停止对这些模型构成挑战.
研究的目的:
- 研究如何在哺乳动物雌性生殖系的长期细胞循环停止期间保持中间体标识,特别是CENP-A染色体.
- 确定是否需要新的CENP-A组件来维持在这种延长停滞期间的中间体,挑战已建立的细胞循环合模型.
主要方法:
- 条件淘汰Mis18α,CENP-A组装机械的关键组件,在雌性生殖线的小鼠.
- 在条件淘汰后对卵细胞中中心基CENP-A核细胞丰度的分析.
- 在雌性小鼠中评估生育能力,具有生殖系特异性的Mis18α淘汰.
主要成果:
- 在雌性生殖系中,Mis18α的有条件淘汰并没有显著影响中心体CENP-A核细胞的丰度.
- 在 I 预相停止期间,长期维持中间体染色质发生的独立于新的 CENP-A 组合.
- 在缺乏生殖系 Mis18α 的小鼠中没有观察到可检测的生育损害,这表明了替代的维持机制.
结论:
- 细胞周期合的CENP-A组件的既定范式在雌性生殖系中长期介质停留期间不严格要求保持中心粒体身份.
- 在雌性哺乳动物中,中间体遗传可能依赖于与连续组装不同的机制.
- 需要进一步的研究,以阐明女性生殖系中基维持的精确机制.
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