长期阅读的ENCODE4RNA-seq集合揭示了不同类型的转录结构多样性的不同类别.
Fairlie Reese1,2, Brian Williams3, Gabriela Balderrama-Gutierrez1,2
1Developmental and Cell Biology, University of California, Irvine, Irvine, USA.
bioRxiv : the preprint server for biology
|June 9, 2023
概括
长时间读取的RNA测序揭示了人类和小鼠的广泛的转录异型多样性. 大多数编码蛋白质的基因在它们的初级转录中表现出变化,突出显示了跨组织和物种的动态基因表达.
科学领域:
- 基因组学就是基因组学.
- 文字转录学 (Transcriptomics) 是一个学科.
- 分子生物学分子生物学
背景情况:
- 哺乳动物基因通常通过使用差异性促销物,替代拼接和多种3'末端处理产生多个转录异型.
- 量化这些多样化的转录是挑战传统的短读RNA测序由于转录长度限制.
结论:
- 这项大规模的LR-RNA-seq调查为了解哺乳动物的替代转录使用提供了基础资源.
- 人类和小鼠 ортолог之间存在显著的转录组差异,尽管在多样性机制上存在全球相似之处.
- 这项研究强调了转录异形表达的动态性质及其进化分歧.
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