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Updated: Jul 27, 2025

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
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2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

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MRT-ModSeq - 使用马拉松RTRT快速检测RNA修饰

Rafael de Cesaris Araujo Tavares1, Gandhar Mahadeshwar2, Han Wan3

  • 1Department of Chemistry, Yale University, New Haven, CT, 06511, USA.

bioRxiv : the preprint server for biology
|June 9, 2023
PubMed
概括

MRT-ModSeq提供了多个RNA修饰的快速,同时检测. 这种新方法使用突变档案准确地绘制了各种RNA修饰,包括m1A,m3U和m7G.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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