用结构化照明显微镜对神经元结构进行超分辨率成像
Tristan C Paul1, Karl A Johnson1, Guy M Hagen1
1UCCS BioFrontiers Center, University of Colorado Colorado Springs, 1420 Austin Bluffs Parkway, Colorado Springs, Colorado, 80918.
bioRxiv : the preprint server for biology
|June 9, 2023
概括
超高分辨率显微镜现在可以对更厚的生物样本进行成像. 这种先进的技术在150微米小鼠大脑部分实现了144纳米分辨率,克服了以前的局限性.
科学领域:
- 光学显微镜是一种光学显微镜.
- 生物医学成像学 生物医学成像学
- 神经科学研究研究的神经科学研究.
背景情况:
- 超分辨率结构化照明显微镜 (SR-SIM) 为生物样品提供高分辨率.
- 由于高空间频率照明,传统的SR-SIM仅限于像培养细胞这样的薄标本.
- 对较厚的组织进行成像对于理解复杂的生物系统至关重要.
结论:
- 修改后的SR-SIM克服了成像厚生物组织的局限性.
- 这一进步扩大了超分辨率显微镜在神经科学和生物医学研究中的适用性.
- 提供了一种用于复杂,较厚的标本高分辨率成像的新方法.
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