婴儿和成年人肠道肠体在形态和功能上是不同的
Grace O Adeniyi-Ipadeola1, Julia D Hankins1, Amal Kambal1,2
1Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX.
bioRxiv : the preprint server for biology
|June 9, 2023
概括
婴儿的人类肠道肠 (HIEs) 与成人HIEs不同,表现出不成熟的肠道特征. 这些婴儿HIE模型对于研究婴儿疾病和药物开发非常有价值.
科学领域:
- 胃肠病学 胃肠病学
- 发育生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 人类肠道肠 (HIEs) 是研究肠道上皮的关键模型.
- 婴儿HIEs在研究中代表性不足,尽管婴儿时期的发育发生了重大变化.
研究的目的:
- 为了确定和描述婴儿的状肌性HIEs.
- 将婴儿HIEs与成人HIEs进行比较,以了解婴儿特有的肠道特征.
- 为了验证婴儿HIEs是否重复婴儿肠表皮的特征.
主要方法:
- 从婴儿外科手术样本中确立的阴性HIEs.
- 使用RNA测序 (RNA-Seq) 和形态分析对婴儿和成人HIEs进行比较.
- 进行功能性研究以验证路径差异和婴儿肠道特征的回顾.
主要成果:
- 在婴儿和成人HIEs之间观察到显著的转录组差异,影响细胞分化,增殖,脂质代谢,先天免疫力和粘附.
- 婴儿HIEs在3D培养中显示出更高比例的肠细胞,杯体和肠内分泌细胞标记物,以及更多的增殖细胞.
- 婴儿HIEs表现出不成熟的胃肠表皮的特征:细胞高度较短,屏障完整性降低,对脊髓灰质炎病毒的先天免疫反应较低.
结论:
- 婴儿HIEs准确地模拟婴儿肠表皮及其独特特征.
- 婴儿HIEs作为婴儿特异性疾病研究和药物发现的有价值的活体模型.
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