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相关概念视频

Protein Folding01:22

Protein Folding

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Overview
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Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

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Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis...
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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular transport, catalysis, and structural support. Proteins have a hierarchical structure comprising at least three levels of organization: primary, secondary, and tertiary structure. Some large proteins have a quaternary structure where individual protein subunits are linked together.
The primary structure of a protein is its amino acid sequence....
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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
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相关实验视频

Updated: Jul 27, 2025

A Protocol for Computer-Based Protein Structure and Function Prediction
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在CASP15中使用MULTICOM增强基于AlphaFold-Multimer的蛋白质复合体结构预测.

Jian Liu1, Zhiye Guo1, Tianqi Wu1

  • 1Department of Electrical Engineering and Computer Science, NextGen Precision Health Institute, University of Missouri, Columbia, MO 65211, USA.

bioRxiv : the preprint server for biology
|June 9, 2023
PubMed
概括

通过改善AlphaFold-Multimer的输入和精制输出,MULTICOM增强了蛋白质复杂结构预测. 这种新的系统在CASP15中获得了最高排名,超过了标准的AlphaFold-Multimer预测.

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科学领域:

  • 计算生物学 计算生物学
  • 结构生物学 结构生物学
  • 生物信息学是一种生物信息学.

背景情况:

  • AlphaFold-Multimer是用于预测蛋白质复杂结构的领先工具.
  • 提高AlphaFold-Multimer的性能需要优化输入数据和改进预测输出.

研究的目的:

  • 开发一个改进的系统 (MULTICOM) 用AlphaFold-Multimer来预测蛋白质四级结构.
  • 为了提高蛋白质复杂结构预测的准确性和可靠性.

主要方法:

  • 开发了MULTICOM系统,集成多种多重序列对齐 (MSA) 和AlphaFold-Multimer的模板.
  • 使用基于Foldseek的结构对齐用于模型生成和改进.
  • 使用多个互补的指标排名结构模型.

主要成果:

  • 在CASP15.15中,MULTICOM系统 (MULTICOM_qa服务器,MULTICOM_人类预测器) 获得了高排名.
  • 与标准AlphaFold-Multimer相比,MULTICOM_qa的TM平均得分增加了5.3%和8%.
  • 基于对齐的模型生成 (FSAMG) 超过了传统的序列对齐方法.

结论:

  • 在蛋白质复杂结构预测方面,MULTICOM显著改进了AlphaFold-Multimer.
  • 该系统的性能通过CASP15竞赛的严格测试来验证.
  • 在预测蛋白质组合的四级结构方面,MULTICOM提供了一个有前途的进步.