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DDX3通过调节外体的分泌和组成来抑制肝细胞癌的进展

Yi-Yuan Su^1,2, Chi-Hong Chao^1,2,3, Hsiang-Yu Hsu^2,3

¹Department of Biological Science and Technology, College of Biological Science and Technology, National Yang Ming Chiao Tung University Hsinchu 300, Taiwan.

American journal of cancer research

|June 9, 2023

概括

在肝细胞癌 (HCC) 中减少DDX3表达促进了外体释放,增强了癌症干性. 这项研究显示了DDX3的存在.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
细胞生物学细胞生物学

背景情况:

由于缺乏早期预测生物标志物和症状,肝细胞癌 (HCC) 的诊断和治疗具有挑战性.
外体细胞在癌症中调解细胞间通信,影响瘤的进展.
DDX3,一个DEAD-boxRNA螺旋酶,被认为是HCC中的瘤抑制剂,但其在外体调节中的作用尚不清楚.

研究的目的:

研究DDX3对HCC细胞外体分泌和货物分类的影响.
阐明DDX3通过外体对HCC进展产生影响的分子机制.

主要方法:

在HCC细胞中抑制DDX3表达.
对外体释放和外体生物发生相关蛋白质 (TSG101,Alix,CD63,Rab蛋白质) 的表达的分析.
在接受HCC细胞中评估癌症干细胞特性 (自我更新,迁移,耐药性).
对外体微RNA含量的分析 (miR-200b,miR-200c).

主要成果:

在HCC细胞中减少DDX3表达显著增加了外体细胞释放和上调的外体细胞生物发生标志物.
来自DDX3-敲击HCC细胞的外体促进了受体细胞中的癌症干细胞特征.
这些外基因组显示出改变的载荷,包括上调的外基因组标记物和下调的抑制瘤的miR-200b和miR-200c.

结论:

在HCC中,DDX3在调节外体分泌和载荷方面发挥着至关重要的作用.
丢失DDX3功能通过改变的外体细胞沟通来增强HCC干性.
这些发现支持DDX3的瘤抑制作用,并建议HCC的潜在治疗点.

关键词:

肝细胞癌是肝细胞癌.它们是RNA基酶DDX3和RNA基酶DDX3.拉布蛋白是拉布蛋白质的一种蛋白质.癌症干细胞是一种癌症干细胞.外基因组是外基因组中的一个.这就是为什么miR-200b.在 miR-200c 中使用.

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