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Aaron Muirhead¹, Kevin Zhu², Joe Brown²

¹Department of Population Health Sciences, School of Public Health, Georgia State University, Atlanta, GA, USA.

Environmental science & technology letters

|June 9, 2023

概括

寨卡病毒 (ZIKV) RNA 在污水中长时间保持可检测性,即使在室温下. 废水监测为追踪ZIKV流行病学提供了一个有希望的,具有成本效益的方法.

科学领域:

环境病毒学环境病毒学
公共卫生监督公共卫生监督
分子流行病学分子流行病学

背景情况:

寨卡病毒 (ZIKV) 感染通常无症状,使流行病学监测复杂化.
有效的监测对于了解ZIKV的传播和影响至关重要.
废水监测为跟踪ZIKV等树状病毒提供了潜在的途径.

研究的目的:

评估检测污水中寨卡病毒RNA的可行性.
为了确定ZIKVRNA在各种温度条件下的污水中的衰变速度.
评估污水监测作为ZIKV流行病学的新工具.

主要方法:

ZIKV (MEX 1-44) 被入原始污水中,并在4°C,25°C和35°C的温度下化一个月.
使用QiaAmp Minelute病毒旋转套件提取病毒RNA.
使用TaqPath寨卡病毒套件量化ZIKVRNA水平.

主要成果:

在研究期间,在4°C时没有观察到ZIKV RNA的显著下降.
在25°C时,可检测的ZIKVRNA的90%衰变发生在大约21天后.
在35°C时,可检测的ZIKVRNA的90%衰变发生在大约8.5天后.

结论:

寨卡病毒RNA在各种温度范围内在污水中相对稳定.
基于废水的监测是监测ZIKV的可行和具有成本效益的战略.
需要对ZIKV流行病学污水监测进行进一步的调查.

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