乳酸细菌具有强烈的抗氧化功能,从"江苏",和便中分离出来
Yue Hu1,2, Yan Zhao3, Xu Jia2,4
1Yan'an Key Laboratory of Microbial Drug Innovation and Transformation, School of Basic Medicine, Yan'an University, Yan'an, Shaanxi, China.
Frontiers in microbiology
|June 9, 2023
概括
来自发酵食品的乳酸细菌 (LAB) 显示出强大的抗氧化特性,保护细胞免受氧化损伤. 菌株J2-5和J2-9被确定为功能性食品和护肤品的有前途的天然抗氧化剂.
科学领域:
- 微生物学与食品科学 微生物学与食品科学
- 生物化学和分子生物学
背景情况:
- 由自由基和铁过载引起的氧化损伤有助于衰老和疾病.
- 开发安全有效的天然抗氧化剂是关键的研究领域.
- 乳酸细菌 (LAB) 具有抗氧化活性,并调节肠道健康.
研究的目的:
- 选和识别具有显著抗氧化能力的乳酸细菌菌株.
- 评估选择的LAB菌株的安全性和益生菌潜力.
- 在细胞模型中评估LAB对氧化损伤的保护作用.
主要方法:
- 选15个LAB菌株使用DPPH,基,超氧化离子激素扫除试验,铁离子化和过氧化耐受性测试.
- 通过疏水性和自我聚合测试对细菌粘附的评估.
- 通过最低抑制度和血液溶解试验进行安全性评估,随后进行16S rRNA识别.
- 使用无细胞超浮体来测试293T细胞对氧化应激的保护作用.
主要成果:
- 五种LAB菌株 (J2-4,J2-5,J2-9,YP-1,W-4) 显示出高的抗氧化活性.
- 乳杆菌发酵素菌株J2-4,J2-5,J2-9被确定为非溶血性菌株.
- 乳酸细菌paracasei菌株YP-1和W-4显示出溶血活性,需要进一步调查.
- 菌株J2-5和J2-9通过增强SOD,CAT和T-AOC活性,在293T细胞中显著保护氧化损伤.
结论:
- 来自"江苏"发酵食品的LAB菌株J2-5和J2-9具有强大的抗氧化和保护性质.
- 这些选定的LAB菌株是作为功能性食品,保健品和护肤配方中的天然抗氧化剂的潜在候选者.
- 对YP-1和W-4等溶血性LAB菌株的安全性进行进一步的研究是有必要的.
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