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Genomics02:02

Genomics

36.5K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
36.5K
Sanger Sequencing01:57

Sanger Sequencing

755.2K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
755.2K
Next-generation Sequencing03:00

Next-generation Sequencing

91.7K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
91.7K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

18.9K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
18.9K
RNA-seq03:21

RNA-seq

10.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.1K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

11.3K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
11.3K

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相关实验视频

Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
10:34

Ultra-long Read Sequencing for Whole Genomic DNA Analysis

Published on: March 15, 2019

22.9K

全基因组测序的完整基因组测序

Valeria Valenzuela Ruiz1, Gustavo Santoyo2, Lorena Jacqueline Gómez Godínez3

  • 1Instituto Tecnológico de Sonora (ITSON), 5 de febrero 818 Sur, C.P. 85000, Col. Centro, Cd. Obregón, Sonora, Mexico.

Current research in microbial sciences
|June 9, 2023
PubMed
概括

植物生长促进细菌Bacillus cabrialesii TE3T的完整基因组被测序. 这项分析确定了农业应用的基因,包括生物控制和抗微生物特性,支持其在可持续农业中的使用.

关键词:
生物控制剂 生物控制剂粮食安全 粮食安全脂质类是类中的一种.促进植物生长的细菌促进了植物生长.整个基因组的测序.

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相关实验视频

Last Updated: Jul 27, 2025

Ultra-long Read Sequencing for Whole Genomic DNA Analysis
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Novel Sequence Discovery by Subtractive Genomics
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An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
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科学领域:

  • 微生物学 微生物学
  • 基因组学就是基因组学.
  • 农业科学 农业科学

背景情况:

  • 卡布里亚莱西菌 (Bacillus cabrialesii TE3T) 是一种被称为促进植物生长和作为生物控制剂的格拉姆阳性细菌.
  • 了解其遗传构成对于优化其农业应用至关重要.

研究的目的:

  • 为了呈现Bacillus cabrialesii TE3T类型菌株的完整的循环化基因组序列.
  • 识别与促进植物生长,生物控制和抗菌活动相关的基因和生物合成集群.

主要方法:

  • 采用了混合基因组组装方法,结合了Illumina MiSeq短读和牛津纳米孔技术 (ONT) MinION长读测序.
  • 基因组注释使用RAST,Prokka (快速原生体基因组注释) 和PGAP (原生体基因组注释管道) 进行.
  • 使用antiSMASH.生物合成基因集群被确定.

主要成果:

  • 一个封闭的圆形染色体的4,125,766 bp与44.2%的G + C含量组装.
  • 该基因组包含4,282个编码DNA序列 (CDS),其中4个与植物生长促进和28个与生物控制有关.
  • 确定了七个假定生物合成基因集群,包括与抗微生物和抗真菌性质相关的素,素和表面素.

结论:

  • 细菌cabrialesii TE3T的完整基因组序列揭示了农业应用的显著遗传潜力.
  • 已识别的基因和集群支持细菌的生物活性,使其成为开发可持续农业的生物基础接种剂的有希望的候选人.