盐度对贝受精前和后发育事件的影响
Rodolf Gabriel Prazeres Silva Lopes1,2, Ana Paula Rego2, Sabrina Melo de Jesus Gomes2
1Departamento de Pesca e Aquicultura, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
Animal reproduction
|June 9, 2023
概括
盐度显著影响卵细胞的发育和受精. 适合Anomalocardia flexuosa繁殖的最佳盐度,特别是生殖囊泡分解和极体挤出,发生在30-35g⋅L-1.
科学领域:
- 水产养殖是水产养殖的一种方式.
- 海洋生物学 海洋生物学
- 生殖生理学 生殖生理学
背景情况:
- 双水产养殖依赖于了解盐度在繁殖中的作用.
- 优化化场生产需要了解对盐度的生理反应.
- 异常心灵 (Anomalocardia flexuosa) 是一个商业上重要的鱼物种.
研究的目的:
- 为了研究不同盐度水平对异常心脏灵性卵子细胞的繁殖过程的影响.
- 为了确定最佳的盐度度,用于前和后受精的发展.
- 为了提供数据来改进三倍体诱导策略.
主要方法:
- 卵细胞被剥离出了Anomalocardia flexuosa. 的卵细胞.
- 卵子被暴露于盐度在15至40g⋅L-1.
- 监测了生殖囊泡分解率 (GVBD) 和极体挤出率 (PB1,PB2).
主要成果:
- 盐度直接影响了GVBD的发病率和卵细胞的稳定性.
- 最佳GVBD (>80%) 发生在30-35g⋅L-1在80-120分钟内.
- 盐度为35g⋅L-1加速PB1 (10分钟) 和PB2 (30分钟) 挤出.
结论:
- 盐度是影响异常心灵病柔性卵子细胞成熟和受精的关键因素.
- 建议通过染色体操纵进行三倍体诱导,盐度为35g⋅L-1.
- 在35g⋅L-1.0的成功三倍体诱导中,受精后冲击的具体时间是至关重要的.
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